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http://dx.doi.org/10.7783/KJMCS.2012.20.6.454

Anti-Microbial and Anti-Wrinkle Effect of Kaempferol and Kaempferol Rhamnosides isolated from Hibiscus cannabinus L.  

Lee, Keun Ha (R&D Center, Morechem Co., Ltd.)
Kong, Hae Jin (R&D Center, Morechem Co., Ltd.)
Cho, Young Long (R&D Center, Morechem Co., Ltd.)
Joo, Chul Gue (R&D Center, Morechem Co., Ltd.)
Kwon, Sun Sang (R&D Center, Morechem Co., Ltd.)
Hwang, Jae Sung (Kyung Hee University, Skin Biotechnology Center)
Park, Chung (R&D Center, Morechem Co., Ltd.)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.6, 2012 , pp. 454-460 More about this Journal
Abstract
In this study, kaempferol and its rhamnosides (${\alpha}$-rhamnoisorobin, afzelin, kaempferitrin) were isolated electively by bioconversion technology from Hibiscus cannabinus L. leaves to evaluate the anti-wrinkles effects and anti-microbial effects. In order to evaluate anti-wrinkles activity, reduction of expression matrix metalloproteinase-1 (MMP-1) protein and proliferation/ pro-collagen production were investigated. Kaempferol and ${\alpha}$-rhamnoisorobin showed inhibition activity of MMP-1 generated to compared to positive control. In HaCaT cell proliferation assay, kaempferol and ${\alpha}$-rhamnoisorobin significantly promoted cell proliferation in a concentration-dependent manner. In addition, procollagen synthesis assays (by HDF-N cell) showd that TGF-${\beta}$ induced procollagen production and also, all four kinds of experimental significantly promoted procollagen synthesis in a concentration-dependent manner. Kaempferol and ${\alpha}$-rhamnoisorobin exhibited strong antimicrobial activities on five of microbes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger.
Keywords
Anti-Microbial; Anti-Wrinkles; Kaempferol; Kaempferol Rhamnosides;
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1 Arakaki PA, Marques MR and Santos MC. (2009). MMP-1 polymorphism and its relationship to pathological processes. Journal of Biosciences. 34:313-320.   DOI
2 Boller S, Soldi C, Marques MC, Santos EP, Cabrini DA, Pizzolatti MG, Zampronio AR and Otuki MF. (2009). Antiinflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation. Journal of Ethnopharmacology. 130:262-266.
3 Choi JW, Kim SI, Kim JY, Yang HJ, Lee KH and Park SN. (2006). Antioxidative and cellular protective effects of Jeju native plant extracts against reactive oxygen species (I). Journal of the Society of Cosmetic Scientists of Korea. 32:181-191.   과학기술학회마을
4 De Melo GO, Malvar Ddo C, Vanderlinde FA, Rocha FF, Pires PA, Costa EA, de Matos LG, Kaiser CR and Costa SS. (2009). Antinociceptive and anti-inflammatory kaempferol glycosides from Sedum dendroideum. Journal of Ethnopharmacology. 12:4228-4232.
5 Fang SH, Rao YK and Tzeng YM. (2005). Inhibitory effects of flavonol glycosides from Cinnamomum osmopholeumon inflammatory mediators in $LPS/IFN-{\gamma}-activatedmurine$ macrophages. Bioorganic & Medicinal Chemistry. 13:2381-2388.   DOI   ScienceOn
6 Navarro-Nunez L, Castillo J, Lozano ML, Martinez C, Benavente-Garcia O, Vicente V and Rivera J. (2009). Thromboxane $A^2$ receptor antagonism by flavonoids: structureactivity relationships. Journal of Agricultural and Food Chemistry. 57:1589-1594.   DOI
7 Im DY and Lee KI. (2011). Antioxidative and antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean Journal of Medicinal Crop Science. 19:238-245.   과학기술학회마을   DOI
8 Jin CW, Eom SH, Park HJ, Ghimeray AK, Yu CY and Cho DH. (2009). Antioxidant activity of Hibiscus cannabinus L. leaves in different growth time. Korean Journal of Medicinal Crop Science. 17:21-25.
9 Jorge AP, Horst H, de Sousa E, Pizzolatti MG and Silva FR. (2004). Insulinomimetic effects of kaempferitrin on glycaemia and on $^{14}C-glucose$ uptake in rats oleusmuscle. Chmico- Biological Interactions. 14:989-996.
10 Lee KH, Cho YL, Joo CG, Joo YJ, Kwon SS and Park C. (2011). Study on the anti-inflammatory effect of kaempferol and kaempferol rhamnosides isolated from Hibiscus cannabinus L. Korean Journal of Medicinal Crop Science. 19:426-434.   과학기술학회마을   DOI   ScienceOn
11 Rothwell JA, Day AJ and Morgan MR. (2005). Experimental determination of octanol-water partition coefficients of quercetin and related flavonoids. Journal of Agricultural and Food Chemistry. 53:4355-4360.   DOI
12 Neena P, Susan A and Raul H. (2011). Beneficial regulation of matrix metalloproteinases for skin health. Enzyme Research. 42:72-85.
13 O'Leary KA, Day AJ, Needs PW, Sly WS, O'Brien NM and Williamson G. (2001). Flavonoid glucuronides are substrates for human liver ?-glucuronidase. FEBS Letters. 503:103-106.   DOI
14 Oikarinen A, Karvonen J, Uitto J and Hannuksela M. (1985). Connective tissue alterations in skin exposed to natural and therapeutic UV-radiation. Photo-Dermatology. 2:15-26.
15 Oikarinen A and Kallioinen M. (1989). A biochemical and immunohistochemical study of collagen in sun-exposed and protected skin. Photo-Dermatology. 6:24-31.
16 Regasini LO, Vellosa JC, Silva DH, Furlan M, de Oliveira OM, Khalil NM, Brunetti IL, Young MC, Barreiro EJ and Bolzani VS. (2008). Flavonols from Pterogyne nitens and their evaluation as myeloperoxidase inhibitors. Phytochemistry. 69:1739-1744.   DOI
17 Tzeng YM, Chen K, Rao YK and Lee MJ. (2009). Kaempferitrin activates the insulin signaling pathway and stimulates secretion of adiponectin in 3T3-L1 adipocytes. European Journal of Pharmacologica Neerlandica. 60:727-734.
18 Ross D, Mendiratta S, Qu ZC, Cobb CE and May JM. (1998). Ascorbate 6-palmitate protects human erythrocytes from oxidative damage. Free Radical Biology & Medicine. 26:81-89.
19 Scharffetter-Kochanek K, Wlaschek M, Briviba K and Sies H. (1993). Singlet oxygen induces collagenase expression in human skin fibroblasts. FEBS Letter. 331 :304-306.   DOI   ScienceOn
20 Seo YC, Choi W Y, Kim JS, Zou Y, Lee YG, Ahn JH, Shin IS and Lee HY. (2010). Enhancement of antimicrobial activity of nano-encapsulated horseradish aqueous extracts against foodborne pathogens. Korean Journal of Medicinal Crop Science. 18:389-397.
21 Tapiero H, Tew KD, Ba GN and Mathe G. (2002). Polyphenols: do they play a role in the prevention of human pathologies? Biomedicine and Pharmacotherapy. 56:200-207.   DOI   ScienceOn
22 Tyrrell RM and Pidoux M. (1989). Singlet oxygen involvement in the inactivation of cultured human fibroblast by UVA and near visible radiations. Photochemistry Photobiology. 49:407-412.   DOI   ScienceOn
23 Zuo Z, Zhang L, Zhou L, Chang Q and Chow M. (2006). Intestinal absorption of hawthorn flavonoids - in vitro, in situ and in vivo correlations. Life Science. 79:2455-2462.   DOI
24 Vishnu Prasad CN, Suma Mohan S, Banerji A and Gopalakrishnapillai A. (2009). Kaempferitrin inhibits GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications. 380:39-43.   DOI
25 Vile GF and Tyrrell RM. (1995). UVA radiation- induced oxidative damage to lipid and protein in vitro and in human skin fibroblasts is dependent on iron and singlet oxygen. Free Radical Biology & Medicine. 18:721-725.   DOI   ScienceOn
26 Wlaschek M, Briviba K, Stricklin GP, Sies H and Scharffetter- Kochanek K. (1995). Singlet oxygen may mediate the ultraviolet A in induced synthesis of interstitial collagenase. The Journal of Ivestigative Dermatology. 104:194-198.   DOI   ScienceOn
27 Zurick KM, Qin C and Bernards MT. (2012). Adhesion of MC3T3-E1 cells bound to dentin phosphoprotein specifically bound to collagen type I. Journal of Biomedical Materials Research. 100:2492-2498.