Browse > Article
http://dx.doi.org/10.7783/KJMCS.2012.20.3.159

Comparison of the Physiological Activities of Extracts from Different Parts of Prunus sargentii  

Yang, Sun-A (Department of Oriental Medicine Materials, Dongshin University)
Cho, Joo-Hyun (Department of Oriental Medicine Materials, Dongshin University)
Pyo, Byoung-Sik (Department of Oriental Medicine Materials, Dongshin University)
Kim, Sun-Min (Department of Oriental Medicine Materials, Dongshin University)
Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.3, 2012 , pp. 159-164 More about this Journal
Abstract
In this study, we investigated the antibacterial activity, antioxidative activity and whitening effect of 75% ethanol extracts from different parts of Prunus sargentii. The total phenolic compound content of the branch extract was 277.92 mg/g as the highest level. In the measurement of DPPH radical scavenging ability, $SC_{50}$ values of the cork layer and branch extract were 26.79 ${\mu}g/m{\ell}$ and 30.13 ${\mu}g/m{\ell}$. In nitric oxide (NO) scavenging ability, $SC_{50}$ values of the branch and leaf extract were 49.19 ${\mu}g/m{\ell}$ and 55.55 ${\mu}g/m{\ell}$. All extracts exhibited higher NO scavenging ability than ascorbic acid used as positive control. On the other hand, in antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus by disc diffusion assay, the pure bark extract showed the highest activity. Moreover, tyrosinase inhibitory activity of cork layer, pure bark and branch extracts showed higher activity than arbutin used as positive control. In the cytotoxicity measurement by MTT assay, leaf extract was exhibited Raw 264.7 cell viabilities of 44.68~61.83% as cytotoxic result in tested concentration. In conclusion, the branch extract of Prunus sargentii will be a functional materials without damage compared to other parts such as pure bark or cork layer in the plant.
Keywords
Prunus sargentii; Antioxidative Activity; Antibacterial Activity; Tyrosinase Inhibitory Activity; Cytotoxicity;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Park JM, Lee JY, Park TS, Park GH, Park KS, Kim TH, Cho YJ, Kwon OJ, Choi KI and An BJ. (2008a). Biological activity investigation, and phenol compounds isolation from barks of Prunus sargentii R. Korean Journal of Medicinal Crop Science. 16:173-182.
2 Park JM, Lee JY, Park TS, Hyun SJ, Kim HH, Cho YJ, Kwon OJ, Son AR, Kim DS and An BJ. (2008b). A study on the cosmeceutical activities of Prunus sargentii R. Journal of the Korean Society for Applied Biological Chemistry. 51:70-78.
3 Park JW. (2011). Antioxidant activity and whitening effect of extracts from different parts of Prunus serrulata var. Spontanea. Kyungnam University. Changwon, Korea. p. 1-2.
4 Park YJ, Kim HJ and Heo BG. (2007). Anti-microbial, antioxidant and anti-inflammation effects with of the flower and the young leaf extracts in oriental cherry plants. Korean Society for People, Plants and Environment. 10:43-49.
5 Radi R, Beckman JS, Bush KM and Freeman BA. (1991). Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide. Journal of Biological Chemistry. 266:4244-4250.
6 Shin MK. (2006). Clinical traditional herbalogy. Younglimsa. Seoul, Korea. p. 399-400.
7 Shin KM, Park YM, Kim IT, Hong SP, Hong JP and Lee KT. (2003). In vitro antiinflammatory activity of amygdalin in murine macrophage Raw 264.7 cells. Korean Journal of Pharmacognosy. 34:223-227.
8 Kim HJ, Heo BK, Baek SH, Park YS and Park YJ. (2006a). Effects of the tea manufacture method on mineral contents and sensory evaluation in flower and young leaf of Prunus serrulata lindl. var. spontanea Max. Korean Society for People, Plants and Environment. 9:26-33.
9 Kim HJ, Park YJ, Bea KS and Heo BG. (2006b). Component analysis of petals and young leaves for oriental cherry plants. Korean Society for People, Plants and Environment. 9:20-25.
10 Lee HJ, Lee SS and Choi DH. (2003). Studies on biological activity of wood extractives(VII)-Antimicrobial and antioxidative activities of extractives from the heartwood of Prunus sargentii. Mokchae Konghak. 31:16-23.
11 Lee HJ, Lee SS, Choi DH and Atsushi K. (2001). Studies on biological activity of wood extractives(VI)-Flavonoids in heartwood of Prunus sargentii. Mokchae Konghak. 29:133-139.
12 Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention : mechanism of action. Journal of Nutrition. 3479S-3485S.
13 Manach C, Williamson G, Morand C, Scalbert A and Remesy C. (2005). Bioavailability and bioefficacy of polyphenols in humans. American Journal of Clinical Nutrition. 81:230S-242S.   DOI
14 Marcocci L, Maguire JJ, Droy-Lefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755.   DOI   ScienceOn
15 Moreno MIN, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Enthropharmacology. 71:109-114.   DOI   ScienceOn
16 Oh YJ, Seo HR, Choi TM and Jung DS. (2010). Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science. 18:373-378.
17 Lee HJ, Lee SS and Choi DH. (2003). Studies on biological activity of wood extractives (7)-antimicrobial and antioxidation activities of extractives from the heartwood of Prunus sargentii. Mokche Konghak. 31:16-23.
18 Otto F and Denis W. (1912). On phosphotungsticphosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
19 Park ES, Shin MK and Song HJ. (1998). A study on the antiallergic effect of Cortex betula Platyphyllae or Cortex pruni Serrulatae extract. Korean Journal of Herbology. 13:57-68.
20 Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200.   DOI   ScienceOn
21 Chung HT, Pae HO, Choi BM, Billiar TR and Kim YM. (2001). Nitric oxide as a bioregulator of apoptosis. Biochemical and Biophysical Research Communications. 282:1075-1079.   DOI   ScienceOn
22 Dewick PM. (2002). Medicinal natural products. Wiley & Sons. Chichester, United Kingdom. p.149-151.
23 Eom HJ, Kim SM, Pyo BS and Lee KI. (2009). Changes of physiological activity by drying temperature in leaf of Eriobotrya japonica. Korean Journal of Pharmacognosy. 40:178-183.
24 Han BH and Han YN. (1978). Immunosuppressant activity of cherry bark extract. Korean Journal of Pharmacognosy. 9:173-175.
25 Im DY and Lee KI. (2011). Antioxidative, antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean Journal of Medicinal Crop Science. 19:238-245.   DOI
26 Jung HA. (2002). Antioxidant constituents from the leaves of Prunus serrurata var. spontanea. Pukyung National University. Busan, Korea. p. 6-16.
27 An BJ, Cho YJ, Son JH, Park JM, Lee JY and Park TS. (2006). Antioxidant effects and tyrosinase inhibition activity of extract of Prunus sargentii Rehder. Journal of the Korean Society for Applied Biological Chemistry. 49:145-148.
28 Kang GJ. (2007). Inhibitory effect of organic extracts from Prunus yedoensis Matsum barks on the atopic dermatitis-like inflammation. Cheju National University. Cheju, Korea. p. 56-60.
29 Jung SW, Lee NK, Kim SJ and Han DS. (1995). Screening of tyrosinase inhibitor from plants. Korean Journal of Food Science and Technology. 27:891-896.
30 Kam WS. (1981). Pharmaceutical botany. National Chinese Medicine Institute. p. 305-306.
31 Kim HJ. (2005). Development of tea using flower and young leaf of oriental cherry(Prunus serrurata var. spontanea). Wonkwang University. Iksan, Korea. p. 11-12.