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http://dx.doi.org/10.7783/KJMCS.2012.20.1.008

Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice  

Jung, Myung-Hwan (College of Veterinary Medicine, Chungbuk National University)
Song, Kyung-Sik (College of Pharmacy, Kyungpook National University)
Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.1, 2012 , pp. 8-15 More about this Journal
Abstract
The present study investigated an ethanol extract of Chaenomeles sinensis fruit (CSF) for possible neuroprotective effects on neurotoxicity induced by amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and also for antidementia activity in mice. Exposure of cultured cortical neurons to $10{\mu}M\;A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $0.1-10{\mu}g/m{\ell}$, CSF inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of mice with 15 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with CSF (10, 25 and 50 mg/kg, p.o. for 7 days) as measured by a passive avoidance test. CSF (50 mg/kg) inhibited the increase of cholinesterase activity in $A{\beta}$ (25-35)-injected mice brain. From these results, we suggest that the antidementia effect of CSF is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that CSF may have a therapeutic role for preventing the progression of Alzheimer's disease.
Keywords
Chaenomeles sinensis Fruit; Neuroprotection; Amyloid ${\beta}$ Protein; Cultured Neurons; Memory Impairment;
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