Browse > Article
http://dx.doi.org/10.7783/KJMCS.2012.20.1.016

Desmutagenic Effect of Water Extract from Areca catechu L. on the Mutagenicity of N-Methyl-N-Nitro-N'-Nitrosoguanidinein in E. coli PQ37  

Wu, Wei Jie (Department of Oriental Medicine Resources, Chonbuk National University)
Ahn, Byung-Yong (Department of Oriental Medicine Resources, Chonbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.1, 2012 , pp. 16-19 More about this Journal
Abstract
The desmutagenic activity of the water extract of Areca catechu L. on the mutagenicity induced by aflatoxin $B_1$ ($AFB_1$), N-methyl-N-nitro-N'-nitrosoguani-dine (MNNG), mitomycin C (MMC) and 4-nitroquinoline 1-oxide (4-NQO) was studied by using the SOS Chromotest with Escherichia coli PQ37. The inhibition rates of water extract of Areca catechu L. at concentration of $100{\mu}g/assay$ were 41.0%, 47%, 46%, and 32% against $AFB_1$, MNNG, MMC and 4-NQO, respectively. The water extract of Areca catechu L. was separated into methanol soluble and methanol insoluble parts. The methanol insoluble part exhibited higher inhibition effect than the methanol soluble part against the mutagenic activities of MNNG. Step-wise fractionation of methanol insoluble part was done to obtain methanol, ethyl acetate and water fractions. Among these fractions, water fraction had the strongest inhibitory effect of 45.0% against mutagenicities of MNNG. The inhibition rates of aqueous fraction of methanol-insoluble from water extracted Areca catechu L. at concentrations of 1.61, 16.13, 161.29 and $322.58{\mu}g/mL$ were 12.0%, 24.0%, 47.5% and 62.0%, respectively. The water fraction showed the inhibitory effects with dose response against the mutagenic activity induced by MNNG.
Keywords
Areca catechu L. MNNG; SOS Chromotest; Desmutagenic Activity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Quillardet P and Hofnung M. (1985). The SOS Chromotest, a colorimetric bacterial assay for genotoxins: Procedures. Mutation Research. 147:65-78.   DOI
2 Quillardet P and Hofnung M. (1987). Induction of the SOS system in a dam-3 mutant: a diagnostic strain for chemicals causing DNA mismatches. Mutation Research. 177:17-26.   DOI
3 Song GS, Ahn BY, Lee KS, Maeng IK and Choi DS. (1997a). Effect of hot water extracts from medicinal plants on the mutagenicity of indirect mutagens. Korean Journal of Food Science and Technology. 29:1288-1294.   과학기술학회마을
4 Song GS, Ahn BY, Maeng IK, Kwon YJ, Han SB and Choi DS. (1997b). Antimutagenicity screening of water extracts from chinese herb with SOS Chromotest with several direct mutagens. Food and Biotechnology. 6:214-218.
5 Su HY, Cherng SH, Chen CC and Lee H. (1995). Emodin inhibits the mutagenicity and DNA adducts induced by 1- nitropyrene. Mutation Research. 329:205-212.   DOI
6 Van W CW, Oliver A, De Miranda CM, Van der Bijl P and Grobler-Rabie AF. (1994). Observations on the effect of areca nut extracts on oral fibroblast proliferation. Oral Pathology & Medicine. 23:145-148.   DOI
7 Waters MD, Brady AL, Stack HF and Brockman HE. (1990). Antimutagenicity profiles for some compounds. Mutation Research. 238:59-64.
8 Ferguson LR. (1994). Antimutagen as cancer chemopreventive agents in the diet. Mutation Research. 307:395-410.   DOI   ScienceOn
9 Gichner T and Veleminsky J. (1988). Mechanisms of inhibition of N-nitroso compounds-induced mutagenicity. Mutation Research. 202:325-334.   DOI
10 Hathcock JN. (1987). Mutagens in cooked foods, Nutritional toxicology. Vol. II, Academic Press Inc. Orlando, USA. p. 57.
11 Hayatsu H, Arimoto S and Negishi T. (1989). Dietary inhibitors of mutagenesis and carcinogenesis. Mutation Research. 202: 429-446.
12 Ho CT, Lee CY and Huang MT. (1992). Phenolic compounds in food and their effects on health. American Chemical Society Symposium. 506:102-117.
13 Kata T, Morita K and Inoue T. (1981). Anti-mutagenic action of vegetable factor(s) on the mutagenic principle of tryptophan pyrolysate. Mutation Research. 53:351-219.
14 Ohta T, Watanabe M, Shirasu Y and Inoue T. (1982). Postreplication repair and recombination in uvrA umuC strains of Escherichia coli are enhanced by vanillin, an antimutagenic compound. Mutation Research. 201:107-112.
15 Kristina S, Yun L, Jagadeesan N, Helmut B, Kristina A and Roland CG. (1989). Cytotoxic and genotoxic effects of Arecarelated compounds in cultured human buccal epithelial cells. Cancer Research. 48:5294-5298
16 Lee KS, Kim SH, Chun SH, Park SS and Shin YS. (1998). Antimicrobial activity of Areca catachu L. extract of against intestinal pathogens. Korean Journal of Food and Nutrition. 11:36-40.   과학기술학회마을
17 Ohta T, Watanabe K, Moriya M and Shirasu Y. (1983). Antimutagenic effects of cinnamaldehyde on chemical mutagenesis in Escherichia coli. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 107:219-227.   DOI
18 Ahn BY, Lee KS, Maeng IK, Song GS and Choi DS. (1998). Bio-antimutagenic effect of water extract from Rehmannia glutinosa Liboschitz in SOS Chromotest. Korean Journal of Food Science and Technology. 30:439-445.
19 Ahn BY, Kim DG and Choi DS. (1999). Antimutagenic effect of Tansen (Salvia miltriorrhiza Bunge). Korean Journal of Microbiology and Biotechnology. 27:197-202.   과학기술학회마을
20 Ahn BY, Maeng IK and Choi DS. (1998). The inhibitory effects of medicinal plants on the mutagenicity of hydrogen peroxide. Journal of Woosuk University (HRDEC). 3:13-22.
21 Ahn BY. (2009). Desmutagenic effect of water extract from Cassia tora L. on the mutagenicity of N-methyl-N-nitro-N'- nitrosoguanidinein in E. coli PQ37. Journal of Food Hygiene and Safety. 24:46-49.