Browse > Article
http://dx.doi.org/10.7783/KJMCS.2011.19.5.373

Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts  

Bak, Jong-Phil (The Clinical Trial Center for Bio-Industry, Semyung University)
Son, Jung-Hyun (The Clinical Trial Center for Bio-Industry, Semyung University)
Kim, Yong-Min (The Clinical Trial Center for Bio-Industry, Semyung University)
Jung, Joon-Hee (Oriental Medicine, Semyung University)
Leem, Kang-Hyun (Oriental Medicine, Semyung University)
Lee, Eun-Yong (Oriental Medicine, Semyung University)
Kim, Ee-Hwa (The Clinical Trial Center for Bio-Industry, Semyung University)
Publication Information
Korean Journal of Medicinal Crop Science / v.19, no.5, 2011 , pp. 373-379 More about this Journal
Abstract
Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.
Keywords
Paeoniae Radix Rubra; iNOS; COX-2; ERK1/2; PGE2;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Wu GJ, Chen TL, Ueng YF and Chen RM. (2008). Ketamine inhibits tumor necrosis factor-$\alpha$ and interleukin-6 gene expressions in lipopolysaccharide-stimulated macrophages through suppression of toil-like receptor 4-mediated c-Jin N terminal kinase phosphorylation and activator protein-1 activation. Toxicology and Applied Pharmacology. 228:105-113.   DOI   ScienceOn
2 Wu KK. (1996). Cyclooxygenase 2 induction: molecular mechanism and pathophysiologic roles. The Journal of Laboratory and Clinical Medicine. 128:242-245.   DOI   ScienceOn
3 Wu SH, Wu DG and Chen YW. (2010). Chemical constituents and bioactivities of plants from the genus Paeonia. Chemistry & Biodiversity. 7:90-104.   DOI   ScienceOn
4 Yoon T, Cheon MS, Kim SJ, Lee AY, Moon BC, Chun JM, Choo BK and Kim HK. (2010). Evaluation of solvent extraction on anti-inflammatory efficacy of Glycyrrhiza uralensis. Korean Journal of Medicinal Crop Science. 18:28-33.
5 Zhan LY, Xia ZY, Chen C and Wang XY. (2006). Effect of Radix Paeoniae Rubra on the expression of HO-1 and iNOS in rats with endotoxin-induced acute lung injury. Chinese Journal of Traumatology. 9:181-186.
6 Sessa WC. (1994). The nitric oxide synthase family of proteins. Journal of Vascular Research. 31:131-143.   DOI   ScienceOn
7 Skidgel RA, Gao XP, Brovkovych V, Rahman A, Jho D, Predescu S, Standiford TJ and Malik AB. (2002). Nitric oxide stimulates macrophage inflammatory protein2 expression in sepsis. Journal of Immunology. 169:2093-2101.   DOI
8 Sun WY, Wei W, Gui SY, Wu L and Wang H. (2008). Protective effect of extract from Paeonia lactiflora and Astragalus membranaceus against liver injury induced by Bacillus Calmette-Guerin and lipopolysaccharide in mice. Basic & Clinical Pharmacology & Toxicology. 103:143-149.   DOI   ScienceOn
9 Tetsuka T, Daphna-Iken D, Miller BW, Guan Z, Baier LD and Morrison AR. (1996). Nitric oxide amplifies interleukin 1- induced cyclooxygenase-1 expression in rat mesangial cells. The Journal of Clinical Investigation. 97:2051-2056.   DOI   ScienceOn
10 Torres SH, De Sanctis JB, de L Briceo M, Hernndez N and Finol HJ. (2004). Inflammation and nitric oxide production in skeletal muscle of type 2 diabetic patient. The Journal of Endocrinology. 181:419-427.   DOI   ScienceOn
11 Williams CS and DuBois RN. (1996). Prostaglandin endoperoxide synthase: why two isoforms? The American Journal of Physiology. 270:G393-G400.
12 Palmer RM and Moncada S. (1989). A novel citrulline-forming enzyme implicated in the formation of nitric oxide by vascular endothelial cells. Biochemical and Biophysical Research Communications. 158:348-352.   DOI   ScienceOn
13 Ridnouur LA, Thomas DD, Donzelli S, Espey MG, Roberts DD, Wink DA and Isenberg JS. (2006). The biphasic nature of nitric oxide responses in tumor biology. Antioxidants & Redox Signaling. 8:1329-1337.   DOI   ScienceOn
14 Rodriguez-Barbero A, Dorado F, Velasco S, Pandiella A, Banas B Lopez-Novoa JM. (2006). TGF-$\beta 1$ induces COX-2 expression and PGE2 synthesis through MAPK and PI3K pathways in human mesangial cells. Kidney International. 70:901-909.   DOI   ScienceOn
15 Schmidt H and Walter U. (1994). NO at work. Cell. 78:919-925.   DOI   ScienceOn
16 Mahut B, Delclaux C, Tillie-Leblond I, Gosset P, Delacour C, Zerah-Lancner F, Harf A and de Blic J. (2004). Both inflammation and remodeling influence nitric oxide output in children with refractory asthma. The Journal of Allergy and Clinical Immunology. 113:252-256.   DOI   ScienceOn
17 Schneider A and Stahl RA. (1998). Cyclooxygenase-2 and the kidney: current status and potential perspectives. Nephrology, dialysis, transplantation:official publication of the European Dialysis and Transplant Association-European Renal Association. 13:10-12.
18 Selkoe DJ. (2001). Alzheimer`s disease: genes, protein, and therapy. Physiological Reviews. 81:741-766.
19 Lowenstein CJ, Hill SL, Lafond-Walker A, Wu J, Allen G and Landavere M. (1996). Nitric oxide inhibits viral replication in murine myocarditis. The Journal of Clinical Investigation. 97:1837-1843.   DOI
20 McGinty A, Foschi M, Chang YW, Han J, Dunn MJ and Sorokin A. (2000). Induction of prostaglandin endoperoxide synthase 2 by mitogen-activated protein kinase cascades. The Biochemical Journal. 352:419-424.   DOI   ScienceOn
21 Nathan CF. (1987). Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. The Journal of Clinical Investigation. 80:1550-1560..   DOI
22 Puckett JL, Taylor RW, Leu SY, Guijon OL, Aledia AS, Galant SP and George SC. (2010). An elevated bronchodilator response predicts large airway inflammation in mild asthma. Pediatric Pulmonology. 45:174-181.   DOI   ScienceOn
23 Palmer RM, Ashton DS and Moncada S. (1988). Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 333:664-666.   DOI   ScienceOn
24 Kim SJ, Park JH, Choi SY, Son KH and Kim KU. (2007). Isolated and identification of biological activity compounds from leaves and stem of Paeonia lactiflora Pallas. Korean Journal of Medicinal Crop Science. 15:6-11.
25 Lipton SA, Choi YB, Pan ZH, Lei SZ, Chen HS, Sucher NJ, Loscalzo J, Singel DJ and Stamler JS. (1993). A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compouds. Nature. 364:626-632.   DOI
26 Kock A, Schwarz T, Kirnbauer R, Urbanski A, Perry P and Ansel JC. (1990). Human keratinocytes are a source for tumor necrosis factor-α: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. Journal of Experimental Medicine. 172:1609-1614.   DOI   ScienceOn
27 Lawrence T, Willoughby DA and Gilroy DW. (2002). Antiinflammatory lipid mediators and insights into the resolution of inflammation. Nature Reviews Immunology. 2:787-795.   DOI   ScienceOn
28 Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Soe JS, Choi JH, Lee, ES, Noh HJ and Kim SY. (2011). Anti-inflammatory activity of medicinal plant extracts. Korean Journal of Medicinal Crop Science. 19:217-226.   DOI
29 Guan Z, Buckman SY, Miller BW, Springer LD and Morrison AR. (1998). Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells. The Journal of Biological Chemistry. 273:28670-28676.
30 Grnblatt E, Mandel S and Youdim MB. (2000). Neuroprotective strategies in Parkinson`s disease using the models of 6- hydroxydopamine and MPTP. Annals of the New York Academy of Sciences. 889:262-273.
31 Hibbs JB, Taintor RR and Vavrin Z. (1987). Macrophage cytotoxicity: role of L-arginine deiminase and imino nitrogen oxidation to nitrite. Science. 235:473-476 .   DOI
32 Jeremy JY, Rowe D, Emsley AM and Newby AC. (1999). Nitricoxide and the proliferation of vascular smooth muscle cells. Cardiovascular Research. 43:580-594.   DOI   ScienceOn
33 Jung HW, Mahesh R, Park JH, Boo YC, Park KM and Park YK. (2010). Bisabolangelone isolated from Ostericum kereanum inhibits the production of inflammatory mediators by downregulation of NF-kappaB and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells. International Immunopharmacology. 10:155-162.   DOI   ScienceOn
34 Calderon-Garciduenas L, Reed W, Maronpot RR, Henríquez- Roldan C, Delgado-Chavez R, Calderon-Garcidueñas A, Dragustinovis I, Franco-Lira M, Aragón-Flores M, Solt AC, Altenburg M, Torres-Jardon R and Swenberg JA. (2004). Brain inflammation and Alzheimer`s-like pathology in individuals exposed to severe air pollution. Toxicologic Pathology. 32:650- 658.   DOI
35 Culbert AA, Skaper SD, Howlett DR, Evans NA, Facci L and Soden PE, Seymour ZM, Guillot F, Gaestel M and Richardson JC. (2006). MAPK-activated protein kinase 2 deficiency in microglia inhibits pro-inflammatory mediator release and resultant neurotoxicity. Relevance to neuroinflammati on in a transgenic mouse model of Alzheimer disease. The Journal of Biological Chemistry. 281:23658-23667.   DOI
36 Chen C, Zhang F, Xia ZY, Lin H and Mo AS. (2008). Protective effect of pretreatment with Radix Paeoniae Rubra on acute lung injury induced by intestinal ischemia/reperfusion in rats. Chinese Journal of Traumatology. 11:37-41.   DOI   ScienceOn
37 Chan SC, Kim JW, Hennerson WR Jr and Hanifin JM. (1993). Altered prostaglandin E2 regulation of cytokine production in atopic dermatitis. Journal of Immunology. 151:3345-3352.
38 Clement-Kruzel S, Hwang SA, Kruzel MC, Dasgupta A and Actor JK. (2008). Immune modulation of macrophage proinflammatory response by goldenseal and Astragalus extracts. Journal of Medicinal Food. 11:493-498.   DOI   ScienceOn
39 Adams V, Yu J, Mobius-Winkler S, Linke A, Weigl C, Hibrich L, Schuler G and Hambrecht R. (1997). Increased inducible nitric oxide synthase in skeletal muscle biopsies from patients with chronic heart failure. Biochemical and Molecular Medicine. 61:152-160.   DOI   ScienceOn
40 Adams DO and Hamiton TA. (1984). The cell biology of macrophage activation. Annual Review of Immunology. 2:283- 318.   DOI   ScienceOn
41 Aderem A. (2003). Phagocytosis and the inflammatory response. The Journal of Infectious Diseases. 187:S340-S345.   DOI
42 Bredt DS and Snyder SH. (1989). Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum. Proceedings of the National Academy of Sciences of the United States of America. 86:9030-9033.   DOI   ScienceOn
43 Bredt DS and Snyder SH. (1994). Transient nitric oxide synthase neurons in embryonic cerebral cortical plate, sensory ganglia, and olfactory epithelium. Neuron. 13:301-313.   DOI   ScienceOn