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http://dx.doi.org/10.7783/KJMCS.2011.19.5.368

Antioxidative and Nitric Oxide Production Inhibitory Activities of Lespedeza bicolor Stem Extracts Depending on Solvents  

Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
Yang, Sun-Ah (Deptartment of Oriental Medicine Materials, Dongshin University)
Kim, Sun-Min (Deptartment of Oriental Medicine Materials, Dongshin University)
Publication Information
Korean Journal of Medicinal Crop Science / v.19, no.5, 2011 , pp. 368-372 More about this Journal
Abstract
In this study, we investigated on antioxidative activity and nitric oxide production inhibitory activity of various solvent extracts of Lespedeza bicolor. The total polyphenol content of the methanol extract was 192.6 mg/g and flavonoid content of the acetone extract was 40.6 mg/g, as the highest content. In DPPH radical scavenging ability, $SC_{50}$ values of the ethanol and methanol extract were exhibited $0.69mg/m{\ell}$ and $0.89mg/m{\ell}$, respectively. However, in nitric oxide(NO) scavenging ability, $SC_{50}$ values of the acetone was exhibited $0.72mg/m{\ell}$ as the highest activity. Moreover, the acetone extract showed strong NO production inhibitory effect in lipopolysaccharide(LPS)-stimulated Raw 264.7 cell. In the cytotoxicity measurement by MTT assay, the extracts were exhibited Raw 264.7 cell viabilities of 92.57~129.04% as nontoxic result in concentration of $65{\sim}650{\mu}g/m{\ell}$. As a result, the acetone extract of L. bicolor could be applicable to functional materials for anti-inflammatory related fields.
Keywords
Lespedeza bicolor; Antioxidative Activity; Nitric Oxide; Cytotoxicity;
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Times Cited By KSCI : 12  (Citation Analysis)
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