Browse > Article
http://dx.doi.org/10.7783/KJMCS.2011.19.5.341

Antioxidant Activities of Rehmannia glutinosa by Traditional Methods  

Kim, Hyo-Jin (Department of Food & Nutrition, Chungnam National University)
Lee, Ji-Yeon (Department of Food & Nutrition, Chungnam National University)
You, Bo-Ram (Department of Food & Nutrition, Chungnam National University)
Doh, Eun-Soo (Department of Oriental Pharmaceutical Science, Joongbu University)
Kim, Mee-Ree (Department of Food & Nutrition, Chungnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.19, no.5, 2011 , pp. 341-346 More about this Journal
Abstract
The objective of this study was to evaluate antioxidant activities of Rehmannia glutinosa (Raw Jihwang) by traditional method. The total phenol content of Rehmannia radix Preparata (the final cycle of Jihwang) was increased to 205%, compared with Rehmannia glutinosa. Antioxidant activities, determined by ferric-reducing antioxidant potential (FRAP), 2,2'-azinobis(3 ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, 1,1-diphenyl-2-picrydrazyl (DPPH) and hydroxyl radical scavenging activities, increased remarkably as the number of steaming-drying cycles increased. Especially, FRAP value increased 285%. Also, $IC_{50}$ values for DPPH and hydroxyl radical scavenging activities of the final 9th-cycling product, decreased 48.4% and 76%, respectively, compared with those of Rehmannia glutinosa. Our result was suggested that antioxidant activities of Rehmannia radix Preparata improve according to the increasing number of steaming-drying cycles.
Keywords
Rehmannia glutinosa; Rehmannia radix Preparata; Steaming-Drying Cycle; Antioxidant Activities;
Citations & Related Records
Times Cited By KSCI : 12  (Citation Analysis)
연도 인용수 순위
1 You BR, Kim HR, Kim HJ, Lee JY, Lee SY, Song MR, Park JY and Kim MR. (2011). Catalpol content an antioxidant activities in various cultivars of Rehmanniae glutinosa. Journal of Korean Society Food Science Nutrition. 40:481-485.   DOI
2 Yu MH, Im HG, Lee HJ, Ji YJ and Lee IS. (2006). Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus juiuba var. inermis rehder. Korean Journal of Food Science Technology. 38:128-134.
3 Pellegrini N, Re R, Yang M and Rice-Evans V. (1998). Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods of Enzymology. 299:379-389.
4 Singleton VL and Rossi JA. (1965). Colorimetry of total phenolics with phosphornolybdic-phosphotungstic acid reagents. American Journal of Enology Viticulture. 16:144-158.
5 Woo KS, Song DS, Lee JS, Lee HB and Jeong HS. (2007). Quality characteristics of Rehmannia radix Preparata with presoaking solvents. Korean Journal of Food Science Technology. 39:289-294.
6 Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY and Chung IM. (2004). Comparison of SOD Activity and phenolics compound contents in various Korean medicinal plants. Korean Journal of Medicinal Crop Science. 12:191-202.
7 Morota T, Sasaki H, Nishimura H, Sungama K, Chin M and Mitsahashi H. (1989). Two iridoid glycosides from Rehmannia glutinosa. Phytochemistry. 28:2149-2153.   DOI   ScienceOn
8 Park BY, Chang SM and Choi J. (1989). Relationships between the inorganic constituents contents and the catalpol and sugar contents in the rhizoma of Rehmannia glutinosa. Journal of Korean Agricultural Chemistry Society. 32:249-254.
9 Park NK, Kim SL, Hur HS and Park CH. (2002). Development of R. radix Preparata with new variety "Jiwhang1". Korean Journal of International Agriculture. 14:3-39.
10 Lee JH, Koh JA, Hwang EY and Hong SP. (2002). Quantitative determination of 5-hydroxymethyl-2-furaldehyde from Rehmanniae radix Preparata according to various processing. Korean Journal of Herbology. 17:145-149.
11 Kim YC, Cho CW, Rhee YK, Yoo KM and Rho JH. (2007). Antioxidant activity of ginseng extracts prepared by enzyme and heat treatment. Journal of Korean Society Food Science Nutrition. 39:1482-1485.
12 Ma JY, Ha CS, Sung HJ and Zee OP. (2002). Hemopoietic effects of rhizoma Rehmanniae Preparata on cyclophosphamideinduced prenicious anemia rats. Korean Journal of Pharmacology. 31:325-334.
13 Kim HJ, Lee JY, You BR, Kim HR, Choi JE, Nam KY, Moon BD and Kim MR. (2011). Antioxidant activities of ethanol extracts from black ginseng prepared by steaming-drying cycles. Journal of Korean Society Food Science Nutrition. 40:156-162.   DOI
14 Kim TH, Kim JM, Beak JM, Kim TW, kim DJ, Park JH and Choe M. (2011). Antioxidant and whitening effects of Agrimonia pilosa Ledeb water extract. Korean Journal of Medicinal Crop Science. 19:177-184.   DOI
15 Chung SK. (1997). Hydroxy radical scavenging effects of species and scavengers from brown mustard. Bioscience Biotechnology and Biochemistry. 61:118-123.   DOI   ScienceOn
16 Lee CK and Seo JM. (2004). Changes of the constituents in the Rehmanniae radix Preaparata during processing. Journal of Korean Society Food Science Nutrition. 33:1748-1752.   DOI
17 Halliwell B and Gutteridge JC. (1984). Oxygen toxicity, radicals, transition metal and disease. Biochemical Journal. 319:1-14.
18 Hwang SY, Hwang BY, Choi WH, Jung HJ, Huh JD, Lee KS and Ro JS. (2001). Quantitative determination of 5- hydroxymethyl-2-furaldehyde in the Rehmanniae radix Preparata samples at various processing stages. Korean Journal of Pharmaconology. 32:116-120.
19 Kang MH, Choi CS, Kim ZS, Chung HK, Min KS, Park CG and Park HW. (2002). Antioxidative activities of ethanol extract prepared from leaves, seed, branch, and aerial part of Crotalaria sessiflora L. Korean Journal of Food Science Technology. 34:1098-1102.
20 Kim DW, Lee YJ, Min WJ, Kim YJ, Rho YD and Yang DC. (2009). Conversion of acidic polysaccharide and phenolics compound of changed ginseng by 9 repetitive steaming and drying process, and its effects of antioxidation. Korean Journal of Oriental Physiology & Pathology. 23:121-126.
21 Cohen G. (1988). Oxygen radicals and Parkinson's disease. In Halliwell B, (ed.). Oxygen Radicals and Tissue Injury. Ferderation of American Societies for Experimental Biology. Bethesda, MD. USA. p. 130-135.
22 Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 26:1199-1200.
23 Doh ES, Chang JP, Lee KH and Seong NS. (2010). Ginsenoside change and antioxidation activity of fermented ginseng. Korean Journal of Medicinal Crop Science. 18:255-265.
24 Cerutti PA. (1985). Peroxidant states and tumor promotion. Science. 227:375-381.   DOI
25 Chun JC, Kim JC, Hwang IT and Kim SE. (2002). Acteoside from Rehmanniae glutinosa nillities paraquat activity in Cucumis sativua. Pesticide Biochemistry and Physiology. 72:153-159.   DOI   ScienceOn
26 Chun JC, Ma SY, Kim SE and Lee HJ. (1997). Physiological responses of Rehmanniae glutinosa to paraquat and its tolerance mechanisms. Pesticide Biochemistry and Physiology. 59:51-63.   DOI   ScienceOn
27 Ahn DK, Kim CM, Shin MK and Lee KS. (1998). Traditional chinese medicine dictionary. Chungdambooks, Seoul. Korea. 2:168-176.
28 Ahn SW, Kim YG, Kim MH, Lee HY and Seong NS. (1999). Comparison of biological activities on Rhemannia Radix and R. Radix Preparata produced in Korea. Korean Journal of Medicinal Crop Science. 7:257-262.
29 Benzie IF and Strain JJ. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power": The FRAP assay. Analytical Biochemistry. 230:70-79.