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http://dx.doi.org/10.7783/KJMCS.2011.19.2.103

Enhancement of Anticancer Activities from Lithospermum erythrorhizon Extracts by Ultra High Pressure Process  

Seo, Yong-Chang (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University)
Choi, Woon-Yong (Department of Biomaterials Engineering, Kangwon National University)
Kim, Ji-Seon (Medical & Bio-Material Research Center and Department of Biomaterials Engineering, Kangwon National University)
Cho, Jeong-Sub (DooSan EcoBizNet)
Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Jin-Chul (Department of Biomaterials Engineering, Kangwon National University)
Lee, Hyeon-Yong (Department of Biomaterials Engineering, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.19, no.2, 2011 , pp. 103-110 More about this Journal
Abstract
This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts ($1.0{\mu}g/m{\ell}$) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding $1.0{\mu}g/m{\ell}$ of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.
Keywords
Lithospermum erythrorhizon; Shikonin; Ultra High Pressure; Anticancer Activities;
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Times Cited By KSCI : 11  (Citation Analysis)
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