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Phylogenic Relationship of Rubus Cultivated in Korea Revealed by Chloroplast DNA Spacers  

Eu, Gee-Suck (Institute of Agricultural Science and Technology, Chonbuk National University)
Park, Myoung-Ryoul (Institute of Agricultural Science and Technology, Chonbuk National University)
Baek, So-Hyeon (National Institute of Crop Science, RDA)
Yun, Song-Joong (Department of Crop Science, Institute of Agricultural Science and Technology, Chonbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.4, 2010 , pp. 266-272 More about this Journal
Abstract
There is a considerable difference in morphological traits between Bokbunja cultivated in Korea (KCB) and Korea native Rubus coreanus, contrary to the conviction that the cultivated Bokbunja is the domestication of R. coreanus. To infer the phylogenetic relationship of KCB with other Rubus species, we compared the chloroplast DNA spacers of KCB with those of several Rubus species including black raspberry, R. occidentalis. The three chloroplast DNA spacers, atpB~rbcL, trnL~trnF, and trnT~trnL, were amplified using the specific primer pairs and converted to Single Strand Conformational Polymorphism (SSCP) markers. The SSCP makers of the chloroplast DNA spacers showed a considerable variation both within and among Rubus species. In the phylogenetic tree generated by the SSCP markers, KCB accessions were located in the same clade with R. occidentalis, but R. coreanus accessions in the different clade. Also, in the phylogenetic tree by the nucleotide sequences of the chloroplast DNA spacer trnL~trnF, KCB located in the same clade with R. occidentalis but not with R. coreanus. These results suggest that the three KCB accessions share higher similarity with R. occidentalis than with R. coreanus in the three chloroplast DNA spacers.
Keywords
Rubus Species; Phylogenetic Relationship; Single Stranded Conformational Polymorphism (SSCP);
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Times Cited By KSCI : 5  (Citation Analysis)
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