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Effects of Water Extract of Glycyrrhiza uralensis on $\beta$-Hexosaminidase Release and Expression of the Cytokines of RBL-2H3 Mast Cells  

Kim, Jeong-Mi (Department of Bio and Health Technology, Kangwon National University)
Kim, Dae-Jung (Department of Bio and Health Technology, Kangwon National University)
Kim, Tae-Hyeuk (Department of Bio and Health Technology, Kangwon National University)
Baek, Jong-Mi (Department of Bio and Health Technology, Kangwon National University)
Kim, Hyun-Sook (Well-being Bioproducts RIC Center, Kangwon National University)
Choe, Myeon (Department of Bio and Health Technology, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.4, 2010 , pp. 231-237 More about this Journal
Abstract
The present study was conducted to investigate the anti-allergic reaction with Glycyrrhiza uralensis. We examined cell viability, $\beta$-hexosaminidase release, IL-4 and IL-13 mRNA expression from RBL-2H3 cell after pre-treatment with 0, 100, 250, 500, 1000${\mu}g/m{\ell}$ of Glycyrrhiza uralensis water extracts. Effects of Glycyrrhiza uralensis on the degranulation and pro-inflammatory cytokines (IL-4 and IL-13) expression were evaluated with $\beta$-hexosaminidase assay, and RT-PCR analysis. We observed that Glycyrrhiza uralensis concentrations from 100${\mu}g/m{\ell}$ to 1000${\mu}g/m{\ell}$ had no effect on cell survival. The release of $\beta$-hexosaminidase decreased significantly with all concentrations of Glycyrrhiza uralensis extracts. The expression of the IL-4 and IL-13 mRNA were decreased by Glycyrrhiza uralensis in dose-dependent manner. These results that Glycyrrhiza uralensis has an anti-histamin effects and controls IL-4, IL-13 secretion on allergic reaction.
Keywords
Glycyrrhiza uralensis; Anti-Allergic; RBL-2H3; IL-4; IL-13; $\beta$-hexosaminidase;
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Times Cited By KSCI : 13  (Citation Analysis)
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