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Optimal Extraction Condition and Characterization of Antidementia Acetylcholinesterase Inhibitor from Job's Tears (Coix lachrymajobi L.)  

Seo, Dong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
Jang, Jeong-Hoon (Dept. of Life Science and Genetic Engineering, Paichai University)
Kim, Na-Mi (KT&G Central Research Institute)
Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.6, 2009 , pp. 434-438 More about this Journal
Abstract
For the development of a new antidementia functional food or alternative drug using agricultural products, Job's tears (Coix lachrymajobi L.), which shows high acetylcholinesterase (AChE) inhibitory activity (55.1%) was selected and the extraction conditions of AChE inhibitor were optimized. AChE inhibitor of Job's tears was maximally extracted when it was treated with 60% methanol at $40^{\circ}C$ for 6 h. The AChE inhibitor of the methanol extracts was partially purified by systematic solvent extraction, thin layer chromatography, silica gel chromatography and reverse-phase HPLC and the partial purified AChE inhibitor with inhibitory activity ($IC_{50}$) of $0.608\;{\mu}g$ was obtained. The partial purified AChE inhibitor was soluble in methanol and hexane, and insoluble in water. Its maximum absorption spectra was 230 nm and also it was stable in the range of $30^{\circ}C$ and $70^{\circ}C$ and pH 4.0-8.0 for 1 h.
Keywords
Antidementia; Acetylcholinesterase Inhibitor; Job's Tears;
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Times Cited By KSCI : 4  (Citation Analysis)
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