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Effect of the BuOH Soluble Fraction of Cinnamomum camphora on Melanin Biosynthesis  

Ha, Sang-Keun (Graduate School of East-West Medical Science, Kyung Hee University)
Moon, Eun-Jung (Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Min-Jae (Graduate School of East-West Medical Science, Kyung Hee University)
Park, Hye-Min (Graduate School of East-West Medical Science, Kyung Hee University)
Yoo, Eun-Sook (Department of Pharmacology, College of Medicine, Cheju National University)
Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Kim, Sun-Yeou (Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.4, 2009 , pp. 293-300 More about this Journal
Abstract
This study was carried out to investigate the effect of Cinnamomum camphora on melanogenesis. The MeOH extract of Cinnamomum camphora inhibited mushroom tyrosinase activity in dose-dependent manner. Moreover, it significantly suppressed the melanin production in melan-a cells at the concentration of $100{\mu}/m{\ell}$. The MeOH extract was partitioned with ethyl acetate, n-butanol and water. Among them, the BuOH soluble fraction exhibited significant inhibitory effect on mushroom tyrosinase. In addition, the BuOH soluble fraction reduced the melanin production in melan-a cells. But, the BuOH soluble fraction had less inhibition effects on melan-a cell originated tyrosinase. So, it was performed western blotting for melanogenic proteins (tyrosinase, tyrosinase-related protein (TRP-2)) using melan-a cells. The BuOH soluble fraction inhibited the protein expression of tyrosinase at the concentration of $100{\mu}/m{\ell}$. The results suggested that the BuOH soluble fraction of C. camphora might be a potent inhibitor of melanin biosynthesis in melan-a cells.
Keywords
Melanin; Tyrosinase; Cinnamomum camphora;
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