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Enhancement of Immuno-modulatory of Centella asiatica L. Urban with Edible Polymer through Nano-encapsulation Process  

Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National Univ.)
Kim, Young (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National Univ.)
Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.)
Hwang, Baik (Department of Biology, Chonnam National Univ.)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.4, 2009 , pp. 257-265 More about this Journal
Abstract
Phosphatidylcholine was used to encapsulate aqueous extracts of Centella asiatica, and its biological activity was compared with another aqueous extracts. Nanoparticle of C. asiatica was made by encapsulation to w/o type spherical liposome which of aqueous extracts seized with oil phase as 78.2 nm average diameter. Cytotoxicity of the nanoparticle was measured on human skin fibroblast cells, CCD-986sk, and showed lower cytotoxicity on 1.0 mg/$m{\ell}$ of highest concentration as 28% than that of another extracts. The nanoparticle showed the highest promotion of human B and T cell growth up to 138% and 135%, respectively, compared to the control. and the NK cell growth was promoted up to 8% higher than the control in proportion to secretion of IL-6 and TNF-$\alpha$ from immune cell growth. Also nanoparticle showed highest inhibition activity of hyaluronidase on 1.0 mg/$m{\ell}$ of highest concentration as 60.5%. It seems that because of enhanced biological application of aqueous extracts on cell through nano-encapsulation process.
Keywords
Centella asiatica L. Urban; Nanoparticle; Immune Activity;
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Times Cited By KSCI : 5  (Citation Analysis)
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