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Phylogenetic Analysis of Schizonepeta Spike on the Basis of DNA Sequences  

Jigden, Baigalmaa (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Myung-Kyum (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Noh, Jong-Hun (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Sun, Hua (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.1, 2009 , pp. 46-53 More about this Journal
Abstract
Schizonepeta spike (Korean name "Hyung-Gae") has been used for oriental medicinal purposes in Korea, China and Japan. In this study, twenty six "Hyung-Gae" samples were collected including nine certified Schizonepeta tenuifolia plants, and seventeen commercially marketed "Hyung-Gae" products. Chloroplast trnL-F and rDNA ITS regions of the "Hyung-Gae" samples were sequenced and used to identify whether the samples were genuine S. tenuifolia or not. As the result, the trnL-F and ITS sequences of all the "Hyung-Gae" samples were shown to be identical and it was proven that commercially available medicinal products "Hyung-Gae" are genuine S. tenuifolia. Phylogenetic tree of S. tenuifolia using the trnL-F sequences was constructed and compared with phylogenetic tree using ITS of rDNA region sequences. In these tree, S. tenuifolia was affiliated in the family Lamiaceae. It is proven that trnL-F and ITS phylogenetic trees are useful to study taxonomic position of S. tenuifolia.
Keywords
Hyung-Gae (Schizonepeta tenuifolia); Phylogenetic Analysis; trnL-F; ITS;
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