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Molecular Characterization of the Perilla frutescens Limonene Gene (PFLS) by Agroinfiltration into Nicotiana benthamiana  

Seong, Eun-Soo (Bioherb Research Institute, Kangwon National University)
Seo, Eun-Won (Bioherb Research Institute, Kangwon National University)
Kim, Hyoung-Seok (Department of Natural Resources and Environment Science, University of Illinois)
Heo, Kweon (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University)
Lee, Ju-Kyung (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University)
Chung, Ill-Min (Department of Applied Life Science, Konkuk University)
Ghimire, Bimal Kumar (Department of Applied Life Science, Konkuk University)
Kim, Myong-Jo (Bioherb Research Institute, Kangwon National University)
Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University)
Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.1, 2009 , pp. 33-38 More about this Journal
Abstract
The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.
Keywords
NbPR1; NbSIP1; Oxidative Stresses; PFLS; Transient Overexpression;
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