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Moutan Cortex Extract Inhibits Amyloid ${\beta}$ Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons  

Kim, Joo-Youn (College of Veterinary Medicine, Chungbuk National University)
Ju, Hyun-Soo (College of Veterinary Medicine, Chungbuk National University)
Ban, Ju-Yeon (Department of Pharmacology, College of Medicine, Kyung Hee University)
Song, Kyung-Sik (College of Agriculture and Life-Sciences, Kyungpook National University)
Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.16, no.6, 2008 , pp. 409-415 More about this Journal
Abstract
Moutan cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), has pharmacological effects such as anti-inflammatory, antiallergic, analgesic and antioxidant activities. We investigated a methanol extract of Moutan cortex for neuroprotective effects on neurotoxicity induced by amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to $10\;{\mu}M\;A{\beta}$ (25-35) for 24 h induced neuronal apoptotic death. Moutan cortex inhibited $10\;{\mu}M\;A{\beta}$ (25-35)-induced neuronal cell death at 30 and $50\;{\mu}g/m{\ell}$, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Moutan cortex inhibited $10\;{\mu}M\;A{\beta}$ (25-35)-induced elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) which were measured by fluorescent dyes. Moutan cortex also inhibited glutamate release into medium induced by $10\;{\mu}M\;A{\beta}$ (25-35), which was measured by HPLC. These results suggest that Moutan cortex prevents $A{\beta}$ (25-35)-induced neuronal cell damage by interfering with the increase of $[Ca^{2+}]_i$, and then inhibiting glutamate release and ROS generation. Moutan cortex may have a therapeutic role in preventing the progression of Alzheimer's disease.
Keywords
Moutan Cortex; Amyloid ${\beta}$ protein (25-35); Neurotoxicity; Alzheimer's Disease; Neuroprotection;
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Times Cited By KSCI : 2  (Citation Analysis)
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