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Antiviral Activity of Zanthoxylum Species against Influenza Virus  

Choi, Hwa-Jung (Korea Research Institute of Bioscience and Biotechnology)
Song, Jae-Hyoung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
Kwon, Dur-Han (Korea Research Institute of Bioscience and Biotechnology)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
Ahn, Young-Joon (School of Agricultural Biotechnology, Seoul National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.16, no.4, 2008 , pp. 273-278 More about this Journal
Abstract
We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10${\mu}g/m{\ell}$ with no citotoxicity (50${\mu}g/m{\ell}$) against all of three viruses. In addition, only oseltamivir showed antiviral activity with $IC_{50}$ of 65.3${\mu}g/m{\ell}$ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (-1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.
Keywords
Influenza virus; Zanthoxylum piperitum; Oseltamivir; Cytotoxicity; Antiviral activity;
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