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Extract of Cedrela sinensis Leaves Protects Neuronal Cell Damage Induced by Hydrogen Peroxide in Cultured Rat Neurons  

Lee, Soon-Bok (College of Veterinary Medicine, Chungbuk National University)
Kim, Ju-Yeon (College of Veterinary Medicine, Chungbuk National University)
Cho, Soon-Ock (College of Veterinary Medicine, Chungbuk National University)
Ban, Ju-Yeon (College of Veterinary Medicine, Chungbuk National University)
Ju, Hyun-Soo (College of Veterinary Medicine, Chungbuk National University)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.6, 2007 , pp. 444-450 More about this Journal
Abstract
Dried leaves from Cedrela sinensis A. Juss. (CS), have been observed to possess various pharmacological activity and contain various antioxidant constituents. The protective effect of ethanol extract of CS on hydrogen peroxide $(H_2O_2)-induced$ neurotoxicity was examined using primary cultured rat cortical neurons in the present study. Exposure of cultured neurons to 100 ${\mu}M\;H_2O_2$ caused a significant neuronal death as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. The addition of CS, over a concentration range of 10 to $50{\mu}g/m{\ell}$, concentration-dependently prevented the $H_2O_2-induced$ neuronal apoptotic death. CS $(50{\mu}g/m{\ell})$ significantly inhibited $H_2O_2-induced$ elevation of the cytosolic $Ca^{2+}$ concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, Fluo-4 AM. CS (30 and $50{\mu}g/m{\ell})$ inhibited glutamate release and generation of reactive oxygen species (ROS) induced by $100{\mu}M\;H_2O_2$. These results suggest that CS may mitigate the $H_2O_2-induced$ neurotoxiciy by interfering with the increase of $[Ca^{2+}]_c$, and then inhibiting glutamate release and generation of ROS in cultured neurons.
Keywords
Cedrela sinensis; neurotoxicity; hydrogen peroxide; neuroprotection; cortical neurons;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Bureau G, Longpre F, Martinoli MG (2007) Resveratrol and quercetin, two natural polyphenols, reduced apoptotic neuronal cell death induced by neuroinflammation. J. Neurosci. Res. (in press)
2 Duffy S, MacViar BA (1996) In vitro ischemia promotes calcium influx and intracellular calcium release in hippocampal astrocytes. J. Neurochem. 16:71-81
3 Kang HS, Chung HY, Son KH, Kang SS, Choi JS (2003) Scavenging effect of Korean medicinal plants on the peroxynitrite and total ROS. Natural product Sci. 9:73-79
4 Koh SB, Ban JY, Lee BY, Seong YH (2003) Protective effects of fangchinoline and tetrandrine on hydrogen peroxide-induced oxidative neuronal cell damage in cultured rat cerebellar granule cells. Planta Medica 69:506-512   DOI   ScienceOn
5 Luo XD, Wu SH, Ma YB, Wu DG (2000) Limonoids and phytol derivatives from Cedrela sinensis. Fitoterapia 71 :492-496   DOI   ScienceOn
6 Park MS, Ban JY, Lee JH, Song KS, Seong YH (2006) Methanol extract of Paeonia japonica root protects cultured rat cortical neurons against oxidative damage induced by hydrogen peroxide. Korean J. Medicinal Crop Sci. 14:70-76   과학기술학회마을
7 Lee ZW, Kweon SM, Kim BC, Leem SH, Shin IC, Kim JH, Ha KS (1998) Phosphatidic acid-induced elevation of intracellular $Ca^{2+}$ is mediated by RhoA and $H_2O_2$ in rat-2 fibroblasts. J. Biol. chem. 273:12710-12715   DOI   ScienceOn
8 Whittemore ER, Loo DT, Watt JA, Cotman CW (1995) A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture. Neuroscience 67:921-932   DOI   ScienceOn
9 Mailly F, Marin P, Israel M, Glowinski J, Premont J (1999) Increase in external glutamate and NMDA receptor activation contribute to $H_{2}O_{2}$-induced neuronal apoptosis. J. Neurochem. 73:1181-1188   DOI
10 Volterra A, Trotti D, Racagni G (1994) Glutamate uptake is inhibited by arachidonic acid and oxygen radicals via two distinct and additive mechanisms. Mol. Pharmacol. 46:986-992
11 Lee IS, Wei C, Thuong PT, Song KS, Seong YH, Bae K (2006) Antioxidant constituents from the leaves of Cedrela sinensis A. Juss. Korean J. Medicinal Crop Sci. 14:267-272   과학기술학회마을
12 Lee JH, Cho SO, Ban JY, Song KS, Seong YH (2007) Neuroprotective effect of Korean Mistletoe extract against damage induced by hydrogen peroxide in cultured rat cortical neurons. Korean J. Medicinal Crop Sci. 15:105-111   과학기술학회마을
13 Lee TB (1985) Illustrated Flora of Korea. Hyangmunsa. Seoul. p. 506
14 Park JC, Hur JM, Park JG, Kim HJ, Kang KH, Choi MR Song HS (2000) Inhibitory effects of various edible plants and flavonoids from the leaves of Cedrela sinensis on human immunodeficiency virus type 1 protease. International J. Food Sci. Nutr. 5:170-173
15 Olanow CW (1993) A radical hypothesis for neurodegeneration. Trends Neurosci. 16:439-444   DOI   ScienceOn
16 Pereira CF, Oliveira CR (2000) Oxidative glutamate toxicity involves mitochondrial dysfunction and perturbation of intracellular $Ca^{2+}$ homeostasis. Neurosci. Res. 37:227-236   DOI   ScienceOn
17 Cho EJ, Yokojawa T, Rhyu DY, Kim HY, Shibahara N, Park JC (2003) The inhibitory effects of 12 medicinal plants and their component compounds on lipid peroxidation. Am. J. Chin. Med. 31:907-917   DOI   ScienceOn
18 Gardner AM, Xu FH, Fady C, Jacoby FJ, Duffey DC, Tu Y, Lichtenstein A (1996) Apoptotic vs nonapoptotic cytotoxicity induced by hydrogen peroxide. Free Radical Bio. Med. 22:73-83   DOI   ScienceOn
19 Halliwell B (1992) Reactive oxygen species and the central nervous system. J. Neurochem. 59:1609-1623   DOI   ScienceOn
20 Edmonds JM, Staniforth M (1998) Toona sinensis: Meliaceae. Cutis's Botanical Magazine 15: 186-193   DOI   ScienceOn
21 Su JYC, Kuffy S, Murphy TH (1999) Reduction of $H_2O_2$- evoked, intracellular calcium increases in the rat N 18- RE- 105 neuronal cell line by pretreatment with an electrophilic antioxidant inducer. Neurosci. Lett. 273:109-112   DOI   ScienceOn
22 Pu F, Mishima K, Irie K, Motohashi K, Tanaka Y, Orito K, Egawa T, Kitamura Y, Egashira N, Iwasaki K, Fujiwara M (2007) Neuroprotective effects of quercetin and rutin on spatial memory impairment in an 8-arm radial maze task and neuronal death induced by repeated cerebral ischemia in rats. J. Pharmacol. Sci. 104:329-334   DOI   ScienceOn
23 Stuart GA (1911) Chinese material medica, vegetable kingdom. Shanghai. p. 100
24 Perry LI (1980) Medicinal plants of east and southeast Asia: attributed properties and uses. MIT press. Cambridge. Mass. p. 263
25 Lee HJ, Ban JY, Seong YH (2005) Blockade of $5-HT_3$ receptor with MDL 72222 and Y25130 reduces hydrogen peroxideinduced neurotoxicity in cultured rat cortical cells. Life Sci. 78:294-300   DOI   ScienceOn
26 Wang H, Joseph JA (2000) Mechanisms of hydrogen peroxideinduced calcium dysregulation in PCI2 cells. J. Neurosci. 28: 1222-1231
27 Ban JY, Cho SO, Jeon SY, Song KS, Bae K, Seong YH (2005) Protective effect of Sanguisorba officinalis L. root on amyloid ${\beta}$protein (25-35)-induced neuronal cell damage in cultured rat cortical neuron. Korean J. Medicinal Crop Sci. 13:219-226