Browse > Article

Antioxidant and Anticancer Activity of Fractions from Picrasma quassioides (D. Don) Benn. Methanolic Extract  

Yin, Yu (School of Biotechnology, Kangwon National University)
Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.5, 2007 , pp. 329-334 More about this Journal
Abstract
The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity ($IC_{50}\;=\;114.01\;{\mu}g/mL$), containing high total phenolics and total flavonoids contents, showed $67.59\;Tan\;{\mu}g/mg$ and $64.95\;Que\;{\mu}g/mg$ respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.
Keywords
Picrasma quassioides (D. Don) Benn.; fractions; methanolic extract; antioxidant activity; anticancer activity and apoptosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Birt DF, Hendrich S, Wang WQ (2001) Dietary agents in can­cer prevention: flavonoids and isoflavonoids. Pharmacol Ther. 90:157-177   DOI   ScienceOn
2 Chen YL, Lin SZ, Chang JY, Cheng YL, Tsai NM, Chen SP, Chang WL, Harn HJ (2006) In vitro and in vivo studies of a novel potential anticancer agent of isochaihulactone on human lung cancer A549 cells. Biochem Pharmacol. 72:308-­319   DOI   ScienceOn
3 Halliwell B (1995) Antioxidant characterization: methodology and mechanism. Biochem Pharmacol. 49: 1341-1348   DOI   ScienceOn
4 Lavia I, Friesemb D, Gereshc S, Hadarb Y, Schwartza B (2006) An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells, Cancer Lett. 244:61-70   DOI   ScienceOn
5 Popiolkiewicz J, Polkowski K, Skierski JS, Mazurek AP (2005) In vitro toxicity evaluation in the development of new anticancer drugs-genistein glycosides. Cancer Lett. 229:67­75   DOI   ScienceOn
6 Tengku Muhammad TS, Hughes TR, Ranki H, Cryer A, Ramji DP (2000) Differential regulation of macrophage CCAAT-Enhancer binding protein isoforms by lipopolysac­charide and cytokines. Cytokine. 12:1430-1436   DOI   ScienceOn
7 Voss C, Eyol E, Berger MR (2005) Identification of potent anti­cancer activity in Ximenia americana aqueous extracts used by African traditional medicine. Toxicol Appl Pharmacol. 12:312-321
8 Yin Y, Heo SI, Jung MJ, Wang MH (2007) Antioxidant Prop­erties of Water Extract from Acorn, J. Appl. Biol. Chem. 50:70-73   과학기술학회마을
9 Yoshikawa K, Sugawara S, Arihara S (1995) Pheny1pro­panoids and other secondary metabolites from fresh fruits of Picrasma Quassioides. Phytocheraistry. 40:253-256   DOI   ScienceOn
10 Zhao MM, Yang B, Wang JS, Li BZ, Jiang YM (2006) Identi­fication of the major flavonoids from pericarp tissues of lychee fruit in relation to their antioxidant activities. Food Chem. 98:539-544   DOI   ScienceOn
11 Wang XJ, Wei YQ, Yuan SL, Liu GJ, Lu YR, Zhang J, Wang WD (2006) Potential anticancer activity of litchi fruit peri­carp extract against hepatocellular carcinoma in vitro and in vivo. Cancer Lett. 239:144-150   DOI   ScienceOn
12 Wang Y, Chan FL, Chen Sh, Leung LK (2005) The plant po1yphenol butein inhibits testosterone-induced proliferation in breast cancer cells expressing aromatase. Life Sci. 77:39-­51   DOI   ScienceOn
13 Lu YR, Foo LY (1997) Identification and quantification of major polyphenols in apple pomace. Food Chem. 59:187­-194   DOI   ScienceOn
14 Wang XJ, Yuan SL, Wang J, Lin P, Lin GJ, Lu YR, Zhang J, Wang WD, Wei YQ (2006) Anticancer activity oflitchi fruit pericarp extract against human breast cancer in vitro and in vivo. Toxicol Appl Pharmacol. 215:168-178   DOI   ScienceOn
15 Halliwell B, Gutteridge JMC (1985) Free radicals in biology and medicine. Oxford University Press. Oxford, 218-313
16 Ching Su New Medical College (ed) (1977) Dictionary of Chi­nese Materia Medica: hanghai Scientific Technological Pub­lishers. Shangai. 1294
17 Le Baila JC, Varnata F, Nicolasb JC, Habriouxa G (1998) Estrogenic and antiproliferative activities on MCF-7 human breast cancer cells by flavonoids. Cancer Lett. 130:209-216   DOI   ScienceOn
18 Lee CL, Yang XT, Wan JMF (2006) The culture duration affects the immunomodu1atory and anticancer effect of polysaccharopeptide derived from Corio Ius versicolor. Enzyme Microb Technol. 38:14-21   DOI   ScienceOn
19 Kogure K, Manabe S, Hama S, Tokumura A, Fukuzawa K (2003) Potentiation of anti-cancer effect by intravenous administration of vesiculated a-tocopheryl hemisuccinate on mouse melanoma in vivo. Cancer Lett. 192:19-24   DOI   ScienceOn
20 Shyu YS, Hwang LS (2002) Antioxidant activity of the crude extract of 1ignan glycosides fromunroasted Burma black ses­ame meal. Food Res. Intern. 35:357-365   DOI   ScienceOn
21 Chiu LCM, Ho TS, Wong EYL, Ooi VEC (2006) Ethyl acetate extract of Patrinia scabiosaefolia downregulates anti-apop­totic Bcl-2/Bcl-XL expression, and induces apoptosis in human breast carcinoma MCF-7 cells independent of caspase-9 activation. J Ethnopharmacol. 105:263-268   DOI   ScienceOn
22 Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55-63   DOI   ScienceOn
23 Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Tengku Muhammad TS (2005) Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carci­noma, T47-D cell line. J Ethnopharmacol. 96:287-294   DOI   ScienceOn
24 Zhang T, Lu H, Shang X, Tian YH, Zheng CY, Wang SW, Cheng HHa, Zhou RJ (2006) Nicotine prevents the apopto­sis induced by menadione in human lung cancer cells. Bio­chem Biophys Res Commun. 342:928-934   DOI   ScienceOn
25 Ju EM, Lee SE, Hwang HJ, Kim JH (2004) Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. Life Sci. 74:1013-1026   DOI   ScienceOn
26 Gordon MH (1990) The mechanism of antioxidant action in vitro. In Hudson (Ed.). Food antioxidants. Elsevier. London. 1-18
27 Kousteni S, Tura-Kockar F, Ramji DP (1999) Sequence and expression analysis of a novel Xenpus laevis cDNA that encodes a protein similar to bacterial and chloroplast riboso­mal protein L24. Gene. 235:13-18   DOI   ScienceOn
28 Pakunlu RI, Wang Y, Saad M, Khandare JJ, Starovoytov V, Minko T (2006) In vitro and in vivo intracellular liposoma1 delivery of antisense oligonucleotides and anticancer drug. J Control Release. 114:153-162   DOI   ScienceOn
29 Schmitt CA (2006) Cellular senescence and cancer treatment. Biochim Biophys Acta. 21: 132-136
30 Slater TF, Sawyer B, Strauli UD (1963) Studies on succinate­tetrazolium reductase system. III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta. 77:383-393   DOI
31 Lin JY, Tang CY (2007) Determination oftotal phenolic and fla­vonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem. 101 :140-147   DOI   ScienceOn
32 Qia LF, Xua ZR (2006) In vivo antitumor activity of chitosan nanoparticles. Bioorg Med Chem Lett. 16:4243-4245   DOI   ScienceOn
33 Athukorala Y, Kim KN, Jeon YJ (2006) Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol. 44:1065-­1074   DOI   ScienceOn