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Neuroprotective Effect of Korean Mistletoe Extract against Damage Induced by Hydrogen Peroxide in Cultured Rat Cortical Neurons  

Lee, Ju-Hyun (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University)
Cho, Soon-Ock (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University)
Ban, Ju-Yeon (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University)
Song, Kyung-Sik (College of Agriculture and Life-Sciences, Kyungpook National University)
Seong, Yeon-Hee (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.2, 2007 , pp. 105-111 More about this Journal
Abstract
The protective effect of ethanol extract of Korean mistletoe (KM; Viscum album coloratum) on hydrogen peroxide $(H_{2}O_{2})-induced$ neurotoxicity was examined in primary cultured rat cortical neurons. $H_{2}O_{2}$ reduced viability of cortical neurons in a concentration-dependent manner. The addition of KM, over a concentration range of 10 to 100 ${\mu}g/ml$, concentration-dependently prevented the $H_{2}O_{2}(100\;{\mu}M)-induced$ neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. KM significantly inhibited $H_{2}O_{2}-induced$ elevation of the cytosolic $Ca^{2+}$ concentration $([Ca^{2+}]_{c})$, which was measured by a fluorescent dye, fluo-4 AM. KM inhibited glutamate release into medium and generation of reactive oxygen species (ROS) induced by $H_{2}O_{2}$. These results suggest that KM may mitigate the $H_{2}O_{2}-induced$ neurotoxiciy by interfering with the increase of $[Ca^{2+}]_{c}$, and inhibiting glutamate release and generation of ROS in cultured neurons.
Keywords
Korean mistloetoe; neurotoxicity; hydrogen peroxide; neuroprotection; cortical neurons;
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