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Effect of M11C (Non-lectin Components) Obtained from Korean Mistletoe on the $IL-1\beta$ Secretion from Mouse Splenocytes  

Jun, Myung-Ha (Department of Immunology, College of Medicine, Konkuk University)
Kang, Tae-Bong (The Institute for Biomedical Research, Handong University)
Chang, Sung-Ho (Department of Immunology, College of Medicine, Konkuk University)
Choi, Wahn-Soo (Department of Immunology, College of Medicine, Konkuk University)
Seong, Nak-Sul (National Crop Experiment Station, RDA)
Her, Erk (Department of Immunology, College of Medicine, Konkuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.1, 2007 , pp. 38-45 More about this Journal
Abstract
Korean mistletoe (Viscum album L) extract has been found to posses immunoregulating activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract activates splenocytes to secret interleukin $1\beta(IL-1\beta)$. The splenocytes were treated with M11C, and then collected the supernatant and cell lysate that were prepared to analyze the level of $IL-1\beta$, using ELISA, immunoblotting, and RT-PCR. Maximum effective dose and time of M11C on $IL-1\beta$ secretion from splenocytes were $200{\mu}g/m\ell$ and 8 hours, respectively. Treatment dose and time for the maximum expression of $IL-1\beta$ mRNA were $200{\mu}g/m\ell$ and 4 hours, respectively. Saccharide degradation enzyme Viscozyme L completely blocked the effect of M11C on $IL-1\beta$ secretion from splenocytes. As the result, among non-lectin components saccharide could be regarded as a main component for $IL-1\beta$ expression from splenocytes.
Keywords
Korean mistletoe (Viscum album L); non-lectin components; M11C; splenocytes; $IL-1\beta$ secretion; $IL-1\beta$ mRNA expression;
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Times Cited By KSCI : 4  (Citation Analysis)
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