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Effect of Korean Mistletoe Extract M11C (Non-lectin Components) on the $TNF-\alpha$ Expression and Secretion from Human Peripheral Blood Monocytes  

Jun, Myung-Ha (Department of Immunology, College of Medicine, Konkuk University)
Kang, Tae-Bong (The Institute for Biomedical Research, Handong University)
Chang, Sung-Ho (Department of Immunology, College of Medicine, Konkuk University)
Choi, Wahn-Soo (Department of Immunology, College of Medicine, Konkuk University)
Seong, Nak-Sul (National Crop Experiment Station, RDA)
Her, Erk (Department of Immunology, College of Medicine, Konkuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.1, 2007 , pp. 30-37 More about this Journal
Abstract
It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis $factor-\alpha$ $(TNF-\alpha)$. To examine the effect of M11C on the production of $TNF-\alpha$ from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the $TNF-\alpha$ sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by $anti-TNF-\alpha$ antibody. These data indicated that MCM contained $TNF-\alpha$, suggesting the $TNF-\alpha$ generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that $TNF-\alpha$ was highly detected in MCM by immunoblotting technique. M11C effect on $TNF-\alpha$ production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of $TNF-\alpha$ mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.
Keywords
Korean mistletoe (Viscum album); non-lectin components; M11C; human peripheral monocyte; L929; $TNF-\alpha$ generation; $TNF-\alpha$ mRNA expression;
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Times Cited By KSCI : 2  (Citation Analysis)
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