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Development of SCAR Marker for Discriminating between Violet Flowered Lines and White Flowered Lines in Chinese Bellflower (Platycodon grandiflorum A.)  

Park, Chun-Geon (National Institute of Crop Science, RDA)
Bang, Kyong-Hwan (National Institute of Crop Science, RDA)
Kim, Ok-Tae (National Institute of Crop Science, RDA)
Jin, Dong-Chun (National Institute of Crop Science, RDA)
Kim, Dong-Hwi (National Institute of Crop Science, RDA)
Sung, Jung-Sook (National Institute of Crop Science, RDA)
Seong, Nak-Sul (National Institute of Crop Science, RDA)
Park, Hee-Woon (National Institute of Crop Science, RDA)
Lee, Sang-Chul (Kyungpook National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.1, 2007 , pp. 1-5 More about this Journal
Abstract
To develop a convenient method for discriminating between violet flowered lines and white flowered lines in Chinese bellflower, RAPD analysis was carried out and SCAR markers were generated. Eighteen specific RAPD bands were obtained from 6 OPERON primer sets. Two out of eighteen RAPD bands were cloned into pGEM-T-Easy vectors and then subjected to the nucleotide sequence analysis. PgR1 and PgR2 DNA fragment, each specific for violet and white flowered lines, consist of 887 bp and 863 bp sequences, respectively. Two SCAR markers were developed from RAPD clones: SPgR1 (355 bp) from PgR1 and SPgR2 (493 bp) from PgR2. One (SPgR2) of these two markers was useful to differentiate between violet flowered lines and white flowered lines in Chinese bellflower.
Keywords
Platycodon grandiflorum; white flowered lines; violet flowered lines; RAPD; SCAR marker; discrimination;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Altschul FS, Thomas L, Madden AA, Schaffer, Jinghui Z, Zheng Z, Web M, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402   DOI
2 Das M, Bhattacharya S, Pal A (2005) Generation and characterization of SCARs by cloning and sequencing of RAPD products: A strategy for species-specific development in Bamboo. Ann. Bot. 95:835-841   DOI   ScienceOn
3 In DS, Kim YC, Bang KH, Chung JW, Kim OT, Hyun DY, Cha SW, Kim TS, Seong NS (2005) Genetic relationships of Panax species by RAPD and ISSR analyses. Korean J. Medicinal Crop Sci. 13:249-253   과학기술학회마을
4 Adam-Blondon AF, Sevignac M, Bannerot H, Dron M (1994) SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean. Theor. Appl. Genet. 88:865-870   DOI   ScienceOn
5 중국약재공사 (1995) 중국상용중약재, 과학출판사., p. 420-428
6 Bang KH, Park CG, Jin DC, Kim HS, Park HW, Park CH, Seong NS (2003) Discrimination of species specific DNA markers using RAPD and AFLP analysis between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz. Korean J. Medicinal Crop Sci. 11:268-273   과학기술학회마을
7 Hu J, van Eysden J, Quiros CF (1995) Generation of DNAbased markers in specific genome regions by two-primer RAPD reactions. PCR Methods Appl. 4:346-351   DOI
8 Murray YHG, Thomson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4326   DOI
9 이창복 (1980) 대한식물도감, 향문사., p. 725
10 Hernandez P, Martin A, Doraco G (1999) Development of SCARs by direct sequencing of RAPD products: A practical tool for the introgression and marker assisted selection of wheat. Mol. Breeding 5:245-253   DOI
11 Lee WB, Choi SY, Kim YS (1993) An application of random amplified polymorphic DNA (RAPD) to systematics of some species Lilium in Korea. Korean J. Plant Tax. 23:35-42   DOI
12 Mabberley DJ (1987) The Plant Book. p. 461
13 Naqvi NI, Chattoo BB (1996) Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast resistance gene in rice. Genome 39:26-30   DOI   ScienceOn
14 Paran I, Michelmore RW (1993) Development of reliable PCR based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85:985-993
15 Bang KH, Sung JS, Park CH, Jin DC, Park CG, Yu HS, Park HW, Seong NS (2004) Discrimination of Atractylodes rhizome white using anatomical characteristics and SCAR markers. Korean J. Medicinal Crop Sci. 12:53-59