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Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban  

Choi, Kyung-Mi (Dept. of Biology and Institute of Plant Resources, Chonnam Nat'l. Univ.)
Hwang, Sung-Jin (Dept. of Oriental Medicine Materials, Dongshin Univ.)
Chung, Sang-Jin (Dep. of Genetic Engineering, Chongju University)
Hwang, Baik (Dept. of Biology and Institute of Plant Resources, Chonnam Nat'l. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.14, no.2, 2006 , pp. 87-91 More about this Journal
Abstract
An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA $17.13\;{\mu}M$ and BA $8.9\;{\mu}M$. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.
Keywords
Centella asiatica; adventitious shoot; organogenesis; histological section;
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