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Effects of Growth Regulators on Shoot Regeneration and Polysaccharide Production of Orostachys japonicus Berger  

Kim, Won-Jung (Division of Forest Science, Gyeongsang National University)
Jung, Hee-Young (Division of Forest Science, Gyeongsang National University)
Min, Ji-Youn (Division of Forest Science, Gyeongsang National University)
Park, Dong-Jin (Division of Forest Science, Gyeongsang National University)
Kim, Yong-Duck (Division of Forest Science, Gyeongsang National University)
Kang, Young-Min (Division of Forest Science, Gyeongsang National University)
Choi, Myung-Suk (Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.12, no.5, 2004 , pp. 391-396 More about this Journal
Abstract
Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. O. japonicus was cultured in media containing various growth regulators and carbon sources. The highest regeneration rate was achieved in 1.0 and $3.0\;mg\;l\;^{-1}$ of 2,4-D concentration, while the lowest was obtained in $10.0\;mg\;l\;^{-1}$ 2,4-D concentration. When different carbone sources were added in the culture medium, plant growth was high in 3% sucrose treatment. The micropropagated shoots were successfully acclimatized in artificial soils and produced comparable amont of polysaccharide compred to parent cultivated plants.
Keywords
Orostachys japonicus; polysaccharides; tissue culture; carbon source; regeneration;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Ahn KS, Sim WS, Kim HM, Han SB, Kim IH (1996) Immunostimulating components from the root of Angelica gigas Nakai. Korean J. Pharmacogn. 27:254-261
2 Kim WJ, Kang YM, Park DJ, Huh GH, Lee BH, Choi MS (2004) Otimal culture conditions for In vitro propagation of Orostachys japonicus and enhancement of polysaccharide production. Korean J. Medicinal Crop Sci. 12(2) : 129-134
3 Shin DY, Lee YM, K HJ (1994) Anatomy and artificial seed propagation in anti-cancer plant Orostachys japonicus A. Berger. Korean J. Crop Sci. 39(2):146-157
4 Kojima Y, Kumazawa Y, Shibukawa N, Otsuka K, Mizunoe K (1980) 小鳥保彦 他. Proc. Symp. WAKAN-YAKU 13, 101
5 Francisco JP, Pablo M, Maritza B, Manuel P (2000) Effect of carbon source and sucrose concentration on growth and hexose accumulation of grape berries cultured in vitro. Plant Cell, Tissue and Organ Culture 61: 37-40   DOI   ScienceOn
6 Ahn KS, Sim WS, Kim HM, Han SB, Kim IH (1998) Immunostimulating polysaccharide from cell culture of Angelica gigas Nakai. Biotechnology Letters 20(1):5-7   DOI   ScienceOn
7 Proma K, Kofi EA, Richard FT (2002) Sugar profile of extracellular polysaccharides from different TremelJaspecies. International J. of Food Microbiology. 79:121-129   DOI   ScienceOn
8 Taiz L, Zeiger E (1991) Plant Physiology. The Benjamin / Cummings Publishing Companym, Redwood City, CA
9 Dubois MC, Gilles KA, Hamilton JK, Rebers PA, Smith FRED (1956) Colorimetric method for determination of sugars and related sbstances. Analytical Chemistry 28(3):350-356   DOI
10 Yamada H, Kiyohara H, Cyong JC, Kojima Y, Kumazawa Y, Otsuka Y (1984b) Studies on polysaccharides from Angelica acutiloba. Part 1. Fractionation and biological properties of polysaccharides. Planta Med. 50, 163-167   DOI   ScienceOn
11 Quaite E, Parker RE, Steer MW (1983) Plant cell extension: structural implications for the origin of the plasma membrane. Plant Cell Environ 6: 429-432   DOI
12 Eapen S, George L (1993) Somatic embryogenesis in peanut: Influence of growth regulators and sugars. Plant Cell Tiss. Org. Cult. 4: 151-156   DOI
13 Choi SD, Nam SH, Yang GJ, Cho MJ, Yang MS (1994) Plant regeneration from the stem tissue of Orostachys japonicus A. Berger. Korean J. Plant Tissue Culture 21(2):65-68
14 Yamada H, Kawaguchi N, Ohmori T, Takeshita Y, Taneya S, Miyazaki T (1984a) Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides. Carbobydrate Res. 125,107-115.   DOI   ScienceOn
15 Yang MS, Choi SU (1992) Some biochemical component changes during the culture of Orostachys japonicus A. Berger. Korean J. Plant Tissue Culture 19(4):209-212
16 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473-497   DOI
17 Kumazawa Y, Mizunoe K, Otsuka Y (1982) Immunostimulating polysaccharides separated from hot water extract of Angelica acutiJoba Kitagawa (Yamato TohkD. Immunology 47,75-83
18 Lazzeri PA, Hildebrand DF, Collins GB (1987) Soybean somatic embryogeneis: Effect of hormone and culture manipulations. plant Cell Tiss. Org. CuI. 190: 197-208
19 Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Process Biochemistry 33(6):671-675   DOI   ScienceOn
20 Park HJ, Young HS, Kim JO, Rhee SH, Choi JS (1991) A study on the chemical constituents of Orostachys japonicus A. Berger. Korean J. Pharmacogn 22(2):78-84
21 Rao SR, GA Ravishankar (2002) Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 20: 101-153   DOI   ScienceOn
22 Cunninghame ME, Hall JL (1985) A quantitative stereological analysis of the effect of indoleacetic acid on the dictyosomes in pea stem epidermal cells. Protoplasma 125: 230-234   DOI