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Genetic variation in pure lines of Panax ginseng based on by RAPD analysis  

Kim, Jin-Hee (College of Agric. & Life Sciences, Div. of Plant Resources, Chungnam National University)
Yuk, Jin-Ah (College of Agric. & Life Sciences, Div. of Plant Resources, Chungnam National University)
Cha, Sun-Kyung (College of Agric. & Life Sciences, Div. of Plant Resources, Chungnam National University)
Kim, Hyun-Ho (Kumsan Agricultural Development & Technology Center)
Seong, Bong-Jae (Kumsan Agricultural Development & Technology Center)
Kim, Sun-Ick (Kumsan Agricultural Development & Technology Center)
Choi, Jae-Eul (College of Agric. & Life Sciences, Div. of Plant Resources, Chungnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.11, no.2, 2003 , pp. 102-108 More about this Journal
Abstract
This experiment was conducted to evaluate the diversity and purity of the Korean ginseng (Panax gjnseng) lines developed by the pure line selection using RAPD markers. Four primer (OPA 19, OPM 11, URP 3 and UBC 98) out of the 48 primer tested produced band which showed within-line polymorphisms at least in one line. Within-line polymorphisms were detected in six lines by OPA 19, in four lines by URP 03, in five lines by OPM 11, and in one line by UBC 98 respectively. Five plants obtained from the commercial cultivar 'Cheonpung' were differentiated using the primers OPA 19 and OPM 11. Five plants obtained from the 'Yeonpung were differentiated using the primer OPM 11. Detection of within-line RAPD polymorphisms might be attributed to the fact that cross pollination appear in P. gjnseng and a long period of three to four years required to reach the reproductive stage thereby delay the process to homozygosity.
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