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Quantification of Icariin Contents in Epimedium koreanum N. by Using a Near Infrared Reflectance Spectroscopy  

Kim, Yong-Ho (Div. of Life Sciences, Soonchunhyang Univ.)
Choi, Byoung-Ryourl (Crop Div. Kyounggi ARES, Hwasong)
Baek, Hum-Young (Jung Woo Pharmacentical Co.)
Lee, Young-Sang (Div. of Life Sciences, Soonchunhyang Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.5, 2002 , pp. 340-343 More about this Journal
Abstract
Near infrared reflectance spectroscopy (NIRS) has become widely accepted for rapid quantitative analysis of components in some crops. Our object was to determine icariin contents in whole plant of Epimedium koreanum by using an NIRS system. Total 150 plant samples previously analyzed by HPLC were scanned by NIRS and 68 samples were selected for calibration and validation equation. A calibration equation calculated by MPLS(modified partial least squares) regression technique was developed and a coefficient of determination in calibration and validation sets were 0.95 and 0.82, respectively. A comparison between NIRS estimation and HPLC value was performed with the remaining samples not included in the calibration and validation sets. Most of samples also showed a positive correlation like a validation set. Our results demonstrate that this developed NIRS equation can be practically used as a mass screening method for rapid quantification of icarin contents in Epimedium koreanum N.
Keywords
NIRS; Epimedium koreanum; icariin; HPLC; MPLS regression;
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