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Micropropagation of Kalopanax $pictus(T_{HUNB}.)\;N_{AKAI}$ by bud cultrue  

Kim, Bong-Kyu (Department of Forest Resources, Konkuk University)
Yi, Yong-Sub (Biotechnology Division, Korea Forest Research Institute)
Ahn, Joong-Hoon (Department of Forest Resources, Konkuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.4, 2002 , pp. 249-252 More about this Journal
Abstract
Several plant growth regulators were examined for on their effect on the in vitro propagation of Kalopanax pictus$(T_{HUNB}.)$ $(N_{AKAI}.)$ on WPM medium. Among the cytokinins tested, BA at $13.32\;{\mu}M$ appeared to be the most effective for multiple axillary shoot formation. Although the addition of $2.89\;{\mu}M\;GA_3$ promoted stem elongation, it produced morphologically abnormal leaves and stems. For rooting of the shoots, $4.9\;{\mu}M$ IBA seemed to be more effective than $2.69\;{\mu}M$ NAA. When the regenerated plants were transferred on artificial mixture containing vermiculite and peat-moss (1 : 1, v/v), 81% of them survived and grew normally.
Keywords
Kalopanax pictus; in vitro; micropropagation;
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1 Daykin A, Scott IM, Francis D, Causton DR (1997) Effect of gibberellin on the cellular dynamics of dwarf pea intemode development. Planta 203 : 526-535   DOI   ScienceOn
2 Kaur K (1998) Plants obtained from Acacia catechu WILLD using mature nodal segments. Plant Cell Reports 17 : 427-429   DOI   ScienceOn
3 Prakash MD, Jeffrey BH (1997) Plant Biochemistry. AcademinPress Inc., California. P 554
4 Shim KK, Ha YM, Lee SJ, Shim KB (1997) Mass propagation of Betula pendula 'Purpurea' through axillary bud culture. J. Kor Soc. Hort. Sci. 38 : 776-782
5 Yeoung YR, Lee MH, Kim BS, Kim HK, Kim JH (2001) Seed germination and softwood cutting technique of Kalopanax pictus Nakai. Korean Journal of Plant Resources 14 : 53-59
6 Kang HS, Lee DK (1998) Site and growth characteristics of Kalopanax septemlobus growing at Mt. joongwang in Pyungchang-gun, Kangwon-do. Journal of Korean Forestry Society. 87 : 483-492
7 Laloue M, Pethe C (1982) Dynamics of cytokinin metabolism in tobacco cells. In : Plant growth substances 1982. Proc. llth Int. Plant Growth Sunst. (ed. PF Wareing pp185-195). New York, Academic Press p 683
8 Tornita M, Kondo K (1991) Factors affecting in vitro plantlet regeneration from axillary buds of Quercus acutissima derived from stump sprouts. Res. Rep. For. Tree Breed. Inst. Japan, pp. 33-41
9 Sun SH, Hall RB (1995) Multiple shoot induction from ex vitro and in vitro derived stem node culture of Populus alba L. X P. grandidentata michx. Korean J. Plant Tissue Culture 22 : 131-135
10 Lloyd G, McCown B (1980) Commercially feasiblemicropropagation mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Prop Soc 30 : 421-427
11 Park, HJ (2001) Kalopanax saponin A is a basic saponin structure for the anti-tumor activity of hederagenin monodesmosides. Planta medica.67: 118-121   DOI   ScienceOn
12 Gerd K (1977) Manual of cultivated broad-leaved tree & shrubs. Timber press pp 198-199