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Anthraquinone Production in Transformed Roots of Rheum undulatum L.  

Hwang, Sung-Jin (Dept. of Food and Biotechnology, Dongshin University)
Pyo, Byoung-Sik (Dept. of Food and Biotechnology, Dongshin University)
Chae, Ho-Zoon (Dept. of Biology, Chonnam National University)
Hwang, Baik (Dept. of Biology, Chonnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.2, 2002 , pp. 88-92 More about this Journal
Abstract
The production of anthraquinone has been dectected in transformed roots of Rheum undulatum L. The effects of medium, initial pH, concentration of sucrose, light irradiation and elicitors on anthraquinone production in transformed roots of Rheum undulatum L. were investigated. The maximum production of anthaquinone was achieved in WPM medium (pH 5.7) supplemented with 6% sucrose, 0.5 mg/l $GA_3$, and 50 mg/l chitosan at 16h light $(16{\mu}mol\;m^{-2}s^{-1})$ condition. Under the optimum conditions, the production of anthraquinone reached to 0.18 mg/g(F.W.) after 8 weeks. The content was estimated about 1.3 times of the level of native roots.
Keywords
Rheum undulatum L. transformed roots; anthraquinone; plant growth regulators; light; chitosan;
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