Browse > Article

The Discrimination of Coisis Semen and Coisis lacrima-jobi Semen by the Random Amplified Polymorphic DNAs and Anatomical Characteristics  

Lee, Mi-Young (Korea Institute of Oriental Medicine)
Im, Seung-Hi (Koyang Cactus Experiment Station, Kyonggi-Do ARES)
Kim, Ho-Kyoung (Korea Institute of Oriental Medicine)
Han, Keong-Sik (Woosuk University)
Choi, Yong-Hyu (Woosuk University)
Ju, Young-Seung (Woosuk University)
Oh, Seung-Eun (KonKuk University)
Ko, Byoung-Seob (Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.1, 2002 , pp. 17-23 More about this Journal
Abstract
The seeds of Coix lachryma-jobi Linne var. mayuen Stapf. are used as dietary food for obesity and diabetes under the names of Yulmu in Korea and Yiyiren(薏苡仁) in China. It is one of the drugs promoting diuresis to eliminate the wetness-evil from the lower warmer in the traditional Korean medicine. According to ancient textbook of the traditional Korean medicine, it should be applied to patients with phlegm and heat, etc. The establishment of the method for the discrimination of Coisis Semen is very important for the quality control of drugs. Random amplified polymorphic DNA(RAPD) analysis and anatomical characteristics were used for the discrimination of Coix lachryma-jobi $Linn\acute{e}$ var. mayuen $S_{TAPF}$. and C. lachryma-jobi $Linn\acute{e}$. In the RAPD analysis with 20 primers, 8 primers gave informative and reproducible bands with the genomic DNA. From the cluster analysis, the genus Coix were divided into two groups at similarity coefficient of 0.863.
Keywords
Anatomy; Coix $lachryma-jobiLinn{\acute{e}}$; Coix $lachryma-jobiLinn{\acute{e}}$ var. mayuen Stapf; RAPD;
Citations & Related Records
연도 인용수 순위
  • Reference
1 朱總選. 1959. 救焉本草. 萬曆 14年(1586年) 刊復刻, 中華書局影 印, 北京 p. 357
2 Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull, 19: 11-15
3 難凌恒雌. 1994. 和漢藥百科圖鑑, 保育社, 東京 p. 306-309
4 陶弘景避. 1955. 本草總集注, 中國古典醫學叢刊, 群聯出版社, 北京 p. 312
5 Hu, J. and C. F. Quiros. 1991. Identification of broccoli and cauliflower cultivars by RAPD markers. The Cell Reports 10 :505-511
6 식품의약품안전청. 1998. 고시 제1998-127호 의약품 등 기준 및 시험방법 제2개정, (주)약업신문 출판국, 서울 p. 1355
7 張貴君主編. 1993. 常用中藥濫定大典 黑龍江科學技術出版社, 上 海 p. 224
8 Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18 :6531-6535   DOI   ScienceOn
9 Roholf F. J. 1989. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System. Exter, New York, USA
10 Torres, A. M., T. MaIan and J. I. Cubero. 1993. Identifying rose cultivars using random ampllified polymorphic DNA markers. Hortscience 28(4) : 333-334
11 吳普等述, 孫星術, 孫漏翼 輯. 1976. 神農本草經. 壽慶 4年 (1799年) 刊本復刻, 商務印書館, 北京 p. 135
12 신민교. 1997. 임상본초학 개정증보판. 영림출판사. 서울 p. 654-655