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http://dx.doi.org/10.12972/kjhst.20160045

Resistance of Cabbage Plants to Isolates of Plasmodiophora brassicae  

Jo, Eun Ju (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Jang, Kyoung Soo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Choi, Yong Ho (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Ahn, Kyoung Gu (Joeun Seeds)
Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Publication Information
Horticultural Science & Technology / v.34, no.3, 2016 , pp. 442-452 More about this Journal
Abstract
Clubroot caused by Plasmodiophra brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To investigate resistance of cabbage to disease caused by P. brassicae isolates, we evaluated development of clubroot on commercial clubroot resistant (CR) and non-CR cultivars, a CR line, and $F_3$ lines from a cross between a CR line and a non-CR line using several isolates of P. brassicae. Four P. brassicae isolates (DJ, HN1, GN1, and YC) were used to measure development of clubroot on 16 non-CR cabbage cultivars that have been commercialized in Korea. Although four P. brassicae isolates induced similar disease severity on non-CR Chinese cabbage, these isolates exhibited different virulence on the cabbage cultivars. The YC isolate was the most virulent, followed by the GN1, HN1, and DJ isolates. Despite differences in virulence of the isolates on the cabbage cultivars, a CR cabbage line 'YCR478' and two CR cabbage cultivars showed high resistance to 12 P. brassicae isolates including DJ, HN1, GN1, and YC. When three isolates (YC, GN1, and DJ) were inoculated onto 107 $F_3$ lines that were derived from a cross between 'YCR478' and a susceptible cabbage line 'C1176', our results showed that 89, 33, and 6 of $F_3$ lines were susceptible to YC, GN1, and DJ isolates, respectively. In aspects of resistance, 6, 36, and 67 of $F_3$ lines exhibited resistant responses to YC, GN1, and DJ isolates, respectively. Taken together, these results suggest that resistance of cabbage to clubroot is likely affected by the virulence of P. brassicae isolates.
Keywords
breeding; clubroot; crucifer crops; race; resistant cultivar;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Jang CS, Piao Z, Park YJ, Lim YP (2001) Development of anther-derived lines resistant to clubroot disease in Chinese cabbage. J Kor Soc Hort Sci 42:689-694 (In Korean)
2 Jo S-J, Shim S-A, Jang KS, Choi YH, Kim J-C, Choi GJ (2011) Resistance of cultivars of Chinese cabbage to Plasmodiophora brassicae isolates of several races collected in Korea. Korean J Hortic Sci Technol 29:610-616 (In Korean)
3 Jo S-J, Shim S-A, Jang KS, Choi YH, Kim J-C, Choi GJ (2014) Resistance of chili pepper cultivars to isolates of Phytophthora capsici. Korean J Hortic Sci Technol 32:66-76 (In Korean). doi:10.7235/hort.2014.13079   DOI
4 Komyoji T, Sugimoto K, Mitani S, Matsuo N, Suzuki K (1995) Biological properties of a new fungicide, fluazinam. J Pestic Sci 20:129-135. doi:10.1584/jpestics.20.129   DOI
5 Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624-626
6 Mitani S, Sugimoto K, Hayashi H, Takii Y, Ohshima T, Matsuo N (2003) Effects of cyazofamid against Plasmodiophora brassicae Woronin on Chinese cabbage. Pest Manag Sci 59:287-293. doi:10.1002/ps.627   DOI
7 Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistance cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur J Plant Pathol 105:327-332. doi:10.1023/A:1008705413127   DOI
8 Lahlali R, Peng G, Gossen BD, McGregor L, Yu FQ, Hynes RK, Hwang SF, McDonald MR, Boyetchko SM (2012) Evidence that the biofungicide Serenade (Bacillus subtilis ) suppresses clubroot on canola via antibiosis and induced host resistance. Phytopathology 103:245-254. doi:10.1094/PHYTO-06-12-0123-R   DOI
9 Landry BS, Lincoln SE, Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409-420. doi:10.1139/g92-061   DOI
10 Buczaki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae : Proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65:295-303. doi:10.1016/S0007-1536(75)80013-1   DOI
11 Diederichsen E, Frauen M, Linders EGA, Hatakeyama K, Hirai M (2009) Status and perspectives of clubroot resistance breeding in crucifer crops. J Plant Growth Regul 28:265-281. doi:10.1007/s00344-009-9100-0   DOI
12 Moriguchi K, Takagi C, Ishii K, Nomura K (1999) A genetic map based RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea . Breed Sci 49:257-265. doi:10.1270/jsbbs.49.257   DOI
13 Nomura K, Minegishi Y, Kimizuka-Takagi C, Fujioka T, Moriguchi K, Shishido R, Ikehashi H (2005) Evaluation of $F_2$ and $F_3$ plants introgressed with QTLs for clubroot resistance in cabbage developed by using SCAR markers. Plant Breed 124:371-375. doi:10.1111/j.1439-0523.2005.01105.x   DOI
14 Piao Z, Ramchiary N, Lim YP (2009) Genetics of clubroot resistance in Brassica species. J Plant Growth Regul 28:252-264. doi:10.1007/s00344-009-9093-8   DOI
15 Cheah LH, Veerakone S, Kent G (2000) Biological control of clubroot on cauliflower with Trichoderma and Streptomyces spp. N Z Plant Prot 53:18-21
16 Chiang MS, Crete R (1970) Inheritance of clubroot resistance in cabbage (Brassica oleracea L. var. capitata L.). Can J Genet Cytol 12:253-256. doi:10.1139/g70-036   DOI
17 Fenwick GR, Griffiths NM, Heaney RK (1983) Bitterness in Brussels sprouts (Brassica oleracea L. var. gemmifera ): The role of 126 glucosinolates and their breakdown products. J Sci Food Agric 34:73-80. doi:10.1002/jsfa.2740340111   DOI
18 The Korean Society of Plant Pathology (KSPP) (2009) Vegetables. In W-G Kim, HM Koo, eds, List of Plant Disease in Korea, Ed 5. KSPP, Suwon, Korea, pp 130-132
19 Rocherieux J, Glory P, Giboulot A, Boury S, Barbeyron G, Thomas G, Manzanares-Dauleux MJ (2004) Isolate-specific and broad spectrum QTLs are involved in the control in Brassica oleracea. Theor Appl Genet 108:1555-1563. doi:10.1007/s00122-003-1580-x   DOI
20 Tanaka S, Fujiyama S, Shigemori S, Nakayama A, Ito S, Kameyama-Iwaki M (1998) Pathogenesis of isolates of Plasmodiophophora brassicae from Japan. (1) Race and pathogenesis in clubroot resistant cultivars. Kyushu Pl Prot Res 44:15-19. doi:10.4241/kyubyochu.44.15   DOI
21 van Poppel G, Verhoeven DT, Verhagen H, Goldbohm RA (1999) Brassica vegetables and cancer prevention. Epidemiology and mechanisms. Adv Exp Med Biol 472:159-168. doi:10.1007/978-1-4757-3230-6_14   DOI
22 Voorrips RE (1995) Plasmodiophora brassicae : Aspects of pathogenesis and resistance in Brassica oleracea . Euphytica 83:139-146. doi:10.1007/BF01678041   DOI
23 Hirai M (2006) Genetic analysis of clubroot resistance in Brassica crops. Breed Sci 56:223-229. doi:10.1270/jsbbs.56.223   DOI
24 Figdore SS, Ferrerira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33-44. doi:10.1007/BF00021723   DOI
25 Grandclement C, Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron. in Brassica oleracea L. Theor Appl Genet 93:86-90. doi:10.1007/BF00225731   DOI
26 Hatakeyama K, Fujimura M, Ishida M, Suzuki T (2004) New classification method for Plasmodiophora brassicae field isolates in Japan based on resistance of $F_1$ cultivars of Chinese cabbage (Brassica rapa L.) to clubroot. Breed Sci 54:197-201. doi:10.1270/jsbbs.54.197   DOI
27 James RV, Williams PH (1980) Clubroot resistance and linkage in Brassica campestris . Phytopathology 70:776-779. doi:10.1094/Phyto-70-776   DOI
28 Yoshikawa H (1993) Studies on breeding of clubroot resistance in cole (Cruciferae) crop. Bull Natl Res Inst Veg Ornam Plants Tea Japan, Ser A, No 7:1-165
29 Voorrips RE, Jongerious MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae ) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75-82. doi:10.1007/s001220050384   DOI
30 Yoshikawa H (1983) Breeding for clubroot resistance of crucifer crops in Japan. Jpn Agr Res Quart 17:6-11