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http://dx.doi.org/10.12972/kjhst.20160001

Vitamin B5 and B6 Contents in Fresh Materials and after Parboiling Treatment in Harvested Vegetables  

Kim, Gi-Ppeum (Department of Herbal Medicine Resource, Kangwon National University)
Ahn, Kyung-Geun (Department of Herbal Medicine Resource, Kangwon National University)
Kim, Gyeong-Ha (Department of Herbal Medicine Resource, Kangwon National University)
Hwang, Young-Sun (Department of Herbal Medicine Resource, Kangwon National University)
Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University)
Choi, Youngmin (Functional Food and Nutrition Division, National Academy of Agricultural Sciences, Rural Development Administration)
Kim, Haeng-Ran (Functional Food and Nutrition Division, National Academy of Agricultural Sciences, Rural Development Administration)
Choung, Myoung-Gun (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Horticultural Science & Technology / v.34, no.1, 2016 , pp. 172-182 More about this Journal
Abstract
This study was aimed to determine the changes in vitamin $B_5$ and $B_6$ contents compared to fresh materials after parboiling treatment of the main vegetables consumed in Korea. The specificity of accuracy and precision for vitamin $B_5$ and $B_6$ analysis method were validated using high-performance liquid chromatography (HPLC). The recovery rate of standard reference material (SRM) was excellent, and all analysis was under the control line based on the quality control chart for vitamin $B_5$ and $B_6$. The Z-score for vitamin $B_6$ in food analysis performance assessment scheme (FAPAS) proficiency test was -1.0, confirming reliability of analytical performance. The vitamin $B_5$ and $B_6$ contents in a total of 39 fresh materials and parboiled samples were analyzed. The contents of vitamin $B_5$ and $B_6$ ranged from 0.000 to 2.462 and from 0.000 to $0.127mg{\cdot}100g^{-1}$, respectively. The highest contents of vitamin $B_5$ and $B_6$ were $2.462mg{\cdot}100g^{-1}$ in fresh fatsia shoots (stem vegetables), and $0.127mg{\cdot}100g^{-1}$ in fresh spinach beet (leafy vegetables), respectively. Moreover, the vitamin $B_5$ and $B_6$ contents for parboiling treatment in most vegetables were reduced or not detected. In particular, the contents of vitamin $B_5$ in parboiled fatsia shoots and vitamin $B_6$ in parboiled yellow potato and spinach beet were decreased 20- and 4-fold compared with fresh material, respectively. These results can be used as important basic data for utilization and processing of various vegetable crops, information for dietary life, management of school meals, and national health for Koreans.
Keywords
analysis method; HPLC/DAD; HPLC/FLD; soluble vitamins; vegetables;
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