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http://dx.doi.org/10.7235/hort.2015.15077

Floral Pigmentation and Expression of Anthocyanin-Related Genes in Bicolored Roses 'Pinky Girl' as Affected by Temporal Heat Stress  

Lee, Seul Ki (Department of Environmental Horticulture, The University of Seoul)
Kim, Wan Soon (Department of Environmental Horticulture, The University of Seoul)
Publication Information
Horticultural Science & Technology / v.33, no.6, 2015 , pp. 923-931 More about this Journal
Abstract
This study was conducted to investigate petal pigmentation and the expression patterns of anthocyanin-related genes in bicolored roses 'Pinky Girl' treated with temporal heat stress (THS). Cyanin accumulation in petals was correlated with floral bud development and started rapidly as floral buds began to open, defined as the $4^{th}$ stage of floral bud development ($S_4$). This stage seems to be most susceptible to petal pigmentation. The total of cyanin pigmentation at blooming was significantly decreased (by 45.5%) with exposure to THS ($39/18^{\circ}C$ for three days at $S_4$) in comparison with control. Meanwhile, the expression of anthocyanin-related genes such as CHS, CHI, F3'H, DFR, ANS, 3GT, and 5GT was relatively promoted by THS. Only F3H was less expressed (by 26.7%) with THS treatment; thus, F3H could be a key gene for bicolor promotion in 'Pinky Girl' among anthocyanin-related genes. Overall, the expression pattern of the most anthocyanin-related genes did not match the trends of cyanin pigmentation in petals. These results suggest that floral pigmentation could be associated with other mechanisms related to anthocyanin biosynthesis such as post-translational effects and regulatory genes.
Keywords
anthocyanin; cyanin; floral bud development; pelargonin;
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1 Burchi, G., D. Prisa, A. Ballarin, and P. Menesatti. 2010. Improvement of flower color by means of leaf treatments in lily. Sci. Hortic. 125:456-460.   DOI
2 Byun, M.S., K.N. Chang, J.S. Kim, M.J. Kim, and K.W. Kim. 2005. Changes tendency of the flower colors in cut Rosa hybrida cultivars. J. Korean Flower Res. Soc. 13:347-353.
3 Christie, P.J., M.R. Alfenito, and V. Walbot. 1994. Impact of lowtemperature stress on general phenylpropanoid and anthocyanin pathways: Enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta 194:541-549.   DOI
4 Dela, G., E. Or, R. Ovadia, A. Nissim-Levi, D. Weiss and M. Oren-Shamir. 2003. Change in anthocyanin concentration and composition in 'Jagure' rose flowers due to transient hightemperature conditions. Plant Sci. 164:333-340.
5 Huh, E.J., H.K. Shin, S.Y. Choi, O.G. Kwon, and Y.R. Lee. 2008. Thermosusceptible developmental stage in anthocyanin accumulation and color response to high temperature in red chrysanthemum cultivars. Korean J. Hortic. Sci. Technol. 26:357-361.
6 Iida, S., Y. Morita, J.D. Choi, K.I. Park, and A. Hoshino. 2004. Genetics and epigenetics in flower pigmentation associated with transposable elements in morning glories. Adv. Biophys. 38:141-159.   DOI
7 Inagaki, Y., Y. Hisatomi, T. Suzuki, K. Kasahara, and S. Iida. 1994. Isolation of a suppressor-mutator/enhancer-like transposable element, Tpn1, from Japanese morning glory bearing variegated flowers. Plant Cell 6:375-383.   DOI
8 Itoh, Y., D. Higeta, A. Suzuki, H. Yoshida, and Y. Ozeki. 2002. Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus). Plant Cell Physiol. 43:578-585.   DOI
9 Jeon, W.H. 2001. Molecular characterization of an UDP-glucose flavonoid glucosyl transferase (ufgt) gene during the colour development in fruits and flowers. MS thesis, Dankook Univ., Korea.
10 Kim, J.H. 1999. The nature of flower color. JinSol Ltd. Seoul, Korea.
11 Kim, W.S. and J.H. Lieth. 2012. Simulation of year-round plant growth and nutrient uptake in Rosa hybrida over flowering cycles. Hortic. Environ. Biotechnol. 53:193-203.   DOI
12 Korea Agro-Fisheries & Food Trade Corporation. 2014. The auction market price of flowers from 2013 to 2014. http://www.yfmc.co.kr.
13 Koes, R., W. Verweij, and F. Quattrocchio. 2005. Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci. 10:236-242.   DOI
14 McGuire, G.R. 1992. Reporting of objective color measurements. Hortic. Sci. 27:1254-1255.
15 Leyva, A., J.A. Jarillo, J. Salinas, and J.M. Marinez-Zapater. 1995. Low temperature induces the accumulation of phenylalanine ammonia-lyase and chalcone synthase mRANs of Arabidopsis thaliana in a light-dependent manner. Plant Physiol. 108:39-46.   DOI
16 Li, Q., J. Wang, H.Y. Sun, and X. Shang. 2014. Flower color patterning in pansy (Viola x wittrockiana Gams.) is caused by the differential expression of three genes from the anthocyanin pathway in acyanic and cyanic flower areas. Plant Physiol. Biochem. 84:134-141.   DOI
17 Longo, L. and G. Vasapollo. 2005. Anthocyanins from bay (Laurus nobilis L.) berries. J. Agric. Food Chem. 53:8063-8067.   DOI
18 Mori, K., S. Sugaya, and H. Gemma. 2005. Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition. Sci. Hortic. 105:319-330.   DOI
19 Murray, JR. and W.P. Hackett. 1991. Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L. Plant Physiol. 97:343-351.   DOI
20 Ogata, J., Y. Kanno, Y. Itoh, H. Tsugawa, and M. Suzuki. 2005. Anthocyanin biosynthesis in roses. Nature 435:757-758.   DOI
21 Quintana, A., J. Albrechtova, R.J. Griesbach, and R. Freyre. 2007. Anatomical and biochemical studies of anthocyanidins in flowers of Anagallis monelli L. (Primulaseae) hybrids. Sci. Hortic. 112:413-421.   DOI
22 Saito, R., N. Fukuta, A. Ohmiya, Y. Itoh, Y. Ozeki, K. Kuchitsu, and M. Nakayama. 2006. Regulation of anthocyanin biosynthesis involved in the formation of marginal picotee petals in Petunia. Plant Sci. 170:828-834.   DOI
23 Vaknin, H., A. Bar-Akiva, R. Ovadia, A. Nissim-Levi, I. Forer, D. Weiss, and M. Oren-Shamir. 2005. Active anthocyanin degradation in Brunfelsia calycina (yesterday-today-tomorrow) flowers. Planta 222:19-26.   DOI
24 Shvarts, M., A. Borochov, and D. Weiss. 1997. Low temperature enhances petunia flower pigmentation and induces chalcone synthase gene expression. Physiol. Plant. 99:67-72.   DOI
25 Stommel, J.R., G.J. Lightbourn, B.S. Winkel, and R.J. Griesbach. 2009. Transcription factor families regulate the anthocyanin biosynthetic pathway in Capsicum annuum. J. Am. Soc. Hortic. Sci. 134:244-251.
26 Sung, S.Y., S.H. Kim, V. Velusamy, Y.M. Lee, B.K. Ha, J.B. Kim, S.Y. Kang, H.G. Kim, and D.S. Kim. 2013. Comparative gene expression analysis in a highly anthocyanin pigmented mutant of colorless chrysanthemum. Mol. Biol. Rep. 40:5177-5189.   DOI
27 Wei, Y.Z., F.C. Hu, G.B. Hu, X.J. Li, X.M. Huang, and H.C. Wang. 2011. Differential expression of anthocyanin biosynthetic genes in relation to anthocyanin accumulation in the pericarp of Litchi chinensis Sonn. PLoS One. 6:e19455.   DOI
28 Weiss, D. 2000. Regulation of flower pigmentation and growth: Multiple signaling pathways control anthocyanin synthesis in expanding petals. Physiol. Plant. 110:152-157.   DOI
29 Yamagishi, M. 2011. Oriental hybrid lily Sorbonne homologue of LhMYB12 regulates anthocyanin biosyntheses in flower tepals and tepal spots. Mol. Breed. 28:381-389.   DOI
30 Zhang, D., B. Yu, J. Bai, M. Qian, Q. Shu, J. Su, and Y. Teng. 2012. Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in 'Yunhongli No. 1' (Pyrus pyrifolia Nakai) pears. Sci. Hortic. 134:53-59.   DOI