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http://dx.doi.org/10.7235/hort.2014.12223

Ethylene Production and Expression of Two Ethylene Biosynthetic Genes in Senescing Flowers of Hosta ventricosa  

Zhu, Xiaoxian (College of Chemistry and Life Sciences, Zhejiang Normal University)
Hu, Haitao (College of Chemistry and Life Sciences, Zhejiang Normal University)
Guo, Weidong (College of Chemistry and Life Sciences, Zhejiang Normal University)
Chen, Jianhua (College of Chemistry and Life Sciences, Zhejiang Normal University)
Wang, Changchun (College of Chemistry and Life Sciences, Zhejiang Normal University)
Yang, Ling (College of Chemistry and Life Sciences, Zhejiang Normal University)
Publication Information
Horticultural Science & Technology / v.32, no.2, 2014 , pp. 261-268 More about this Journal
Abstract
Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.
Keywords
1-aminocyclopropane-1-carboxylic acid (ACC) synthase; ACC oxidase; expression analysis; gene cloning;
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