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http://dx.doi.org/10.7235/hort.2013.12166

A Simple and Fast Microplate Method for Analysis of Carotenoids Content in Chili Pepper (Capsicum annuum L.)  

Hong, Sun Chul (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, Seoul National University)
Han, Jung-Heon (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, Seoul National University)
Lee, Jundae (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, Seoul National University)
Ahn, Yul Kyun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Yang, Eun-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Chae, Soo Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Su (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Yoon, Jae Bok (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, Seoul National University)
Publication Information
Horticultural Science & Technology / v.31, no.6, 2013 , pp. 807-812 More about this Journal
Abstract
In this study, we aimed to develop a simple analysis method for measuring the carotenoids content of pepper powder. A 96-wells polystyrene microplate and an ELISA reader were used for analysis. Although ELISA reader with 450 nm filter was applicable to measure carotenoid contents, the surface of microplates were degenerated by acetone used for carotenoids extraction. However, ten-folded dilute of the color extract with methanol did not affect the surface of polystyrene microplate and components of the color extract could be successfully measured by a ELISA reader, showing a high corelation with ASTA-20.1 method. In addition, this method uses 10 fold less acetone than ASTA-20.1 method resulting less acetone waste. The microplate method using ELISA reader has potential power for analyzing a large number of samples which may be very useful to the practical breeding program for high-colored peppers.
Keywords
breeding; quality; selection;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 American Spice Trade Association (ASTA). 1986. Official analytical method. 2nd ed. ASTA, Englewood Cliffs, NJ. USA. p. 68.
2 Chung, J.H. 1994. Studies on the Accumulation pattern of quality-related compounds in Capsicum Species and its genetic control. Ph.D. Diss., Seoul National University, Seoul, Korea.
3 Commission Internationale de L'Eclairage (CIE). 1986. Colorimetry-publication CIE 15.2. 2nd ed. CIE, Vienna, Austria.
4 Cooper, D.A. 2004. Carotenoids in health and disease: Recent scientific evaluations, research recommendations and the consumer. J. Nutr. 134:221S-224S.
5 Curl, A.L. 1962. The carotenoids of red bell peppers. Agric. Food Chem. 10:504-509.   DOI
6 Giuffrida, D., P. Dugo, G. Dugo, G. Torre, and L. Mondello. 2011. Analysis of native carotenoid composition of sweet bell peppers by serially coupled C30 columns. Nat. Prod. Commun. 6:1817-1820.
7 Gregory, G.K., T.-S. Chrn, and T. Philip. 1987. Quantitative analysis of carotenoids and carotenoid esters in fruits by HPLC: Red bell peppers. J. Sci. 52:1071-1073.
8 Han, J.H., S.C. Hong, J.D. Lee, and J.B. Yoon. 2011. Patent-10-2011-0113163.
9 Jung, J.K., S.J. Lee, S.W. Park, W.G. Kim, and S.I. Moon. 2008. Characteristics of taste on red pepper powder products in Daegu and Gyeongbuk area. Rept. GyeongSang Bukdo Govt. Public Inst. Health Environ. 20:39-58.
10 Lee, H.D., M.H. Kim, and C.H. Lee. 1992. Relationships between the taste components and sensory preference of Korean red peppers. Korean J. Food Sci. Technol. 24:266-271.   과학기술학회마을
11 Mozaffarieh, M., S. Sacu, and A. Wedrich. 2003. The role of the carotenoids, lutein and zeaxanthin, in protecting against age-related macular degeneration: A review based on controversial evidence. Nutr. J. 2:20-27.   DOI   ScienceOn
12 Kweon, O.Y., Y.K. Kim, J.B. Yoon, H.G. Park, and W.M. Yoon. 2006. Inheritance of carotenoids content in red pepper (Capsicum annuum L.) fruit. Kor. J. Hort. Sci. Technol. 24:123-127.   과학기술학회마을
13 Snodderly, D.M. 1995. Evidence for protection against age-related macular degeneration by carotenoids and antioxidant vitamins. Am. J. Clin. Nutr. 62(Suppl.):1448s-1460s.