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http://dx.doi.org/10.7235/hort.2013.13057

Development of Sequence Characterized Amplified Region Markers for Cultivar Identification in Persimmon  

Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Cho, Kwang-Sik (Pear Experiment Station, National Institute of Horticultural & Herbal Science)
Han, Jeom Hwa (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Chun, Jae An (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Hwang, Hae-Sung (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Publication Information
Horticultural Science & Technology / v.31, no.6, 2013 , pp. 798-806 More about this Journal
Abstract
Precise, fast, and cost-effective identification of crop cultivars is essential for plant breeder's rights. Traditional methods for identification of persimmon cultivars are based on the evaluation of sets of morphological characteristics. However, it is difficult to distinguish closely related cultivars using only morphological traits. This study was conducted to develop DNA markers for identification of the 32 persimmon cultivars in Korea and Japan. A total of 309 randomly amplified polymorphic DNA (RAPD) markers were identified using 40 different random primers. Various number of polymorphic bands ranged from 4 (OPP-08) to 14 (UBC159) were detected with an average of 7.7. Resulting 57 RAPD fragments were selected, and their sequences were determined for developing sequence characterized amplified region (SCAR) markers. As a result, 15 of 57 RAPD fragments were successfully converted to SCAR markers. Single polymorphic bands of the same size as or smaller than the RAPD fragments were amplified depending on SCAR markers. Among these markers, a combination of eight SCAR markers (PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, and PS631_735) provided sufficient polymorphisms to identify 32 persimmon cultivars. These newly developed markers will be a fast and reliable tool to identify persimmon cultivars.
Keywords
Diospyros kaki; polymorphism; RAPD; sequence;
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