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http://dx.doi.org/10.7235/hort.2013.12169

Construction of Tomato yellow leaf curl virus Clones for Resistance Assessment in Tomato Plants  

Choi, Seung Kook (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Choi, Hak Soon (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Yang, Eun Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cho, In Sook (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cho, Jeom Deog (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Chung, Bong Nam (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Horticultural Science & Technology / v.31, no.2, 2013 , pp. 246-254 More about this Journal
Abstract
Five isolates of Tomato yellow leaf curl virus (TYLCV) collected from various regions of Korea were amplified using PCR and determined the sequences of full-length genome, respectively. The PCR-amplified DNA of each TYLCV isolate was introduced into a binary vector to construct infectious clone containing 1.9 copies of the corresponding viral genome. Various cultivars and breeding lines of tomato were inoculated with Agrobacterium tumefaciens harboring infectious clone of each TYLCV isolate to assess resistance against TYLCV. Susceptible cultivar 'Super-sunread' revealed typical yellowing and narrowing of the upper leaves. In contrast, breeding linesTY12, GC9, GC171, and GC173, which contained the TY-1 and/or TY-3 genes that confer resistance against TYLCV in nature, were completely symptomless, suggesting that the lines were resistant to challenging TYLCV isolates. Symptoms of TYLCV in susceptible tomato cultivars are significantly different from those of TYLCV in the resistant tomato cultivars at 30 days after agroinfiltration. Although genomic DNAs of TYLCV were detected from the breeding lines TY12, GC9, GC171, and GC173 using real-time PCR analysis with specific primers, levels of TYLCV DNA accumulation in the resistant breeding lines were much lower than those of TYLCV DNA accumulation in susceptible tomato cultivars. Similar symptom severity and levels of TYLCV DNA accumulation were observed from TYLCV infections mediated by Bemisia tabaci in the resistant and susceptible tomato cultivars. Concentration of agrobacterium did not affect the response of tomato cultivars against TYLCV inoculation. Taken together, these results suggest that TYLCV inoculation via agroinfiltration is as effective as inoculation through Bemisia tabaci and is useful for breeding programs of TYLCV-resistant tomato.
Keywords
agroinfiltration; Lycopericum esculentum; real-time PCR; susceptible; symptom;
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