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Effects of Extraction Condition on Extraction Efficiency of Rubiadin in Adventitious Roots of Noni (Morinda citrifolia)  

Kim, Myong-Ki (Department of Horticulture, Chungbuk National University)
Jeong, Cheol-Seung (Health Industry Center, Chungbuk Technopark)
Shin, Yong-Kook (Health Industry Center, Chungbuk Technopark)
Park, Kyong-Hee (Health Industry Center, Chungbuk Technopark)
Lee, Woon-Jang (Health Industry Center, Chungbuk Technopark)
Lee, Eun-Jung (CBN PLANTECH)
Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Publication Information
Horticultural Science & Technology / v.28, no.4, 2010 , pp. 685-690 More about this Journal
Abstract
Rubiadin, a major compound of noni ($Morinda$ $citrifolia$) adventitious root, is highly valued in pharmaceutical industry due to hepatoprotective activity. To dissect rubiadin's effective extraction condition, extraction process of noni adventitious roots was performed with different solvent types, ratio of water to methanol (water, 20, 40, 60, 80, and 100% of methanol), extraction time, and extraction method. In contrast, we also developed a reverse- phase HPLC assay method to determine rubiadin from noni adventitious roots. The HPLC assay of rubiadin was performed by C-18 column using a gradient solvent system of methanol and water with UV detector at 280 nm. The extraction efficiency of different types of solvents were increased in order of methanol (0.08%) > ethanol (0.05%) > acetonitrile (0.03%) > acetone (0.02%) and methylene chloride (0.02%). The results of rubiadin extraction using different solvents showed that 1 hour of ultrasonic extraction was effective in order of 60% methanol (0.21%) > 80% methanol (0.13%) > 100% methanol (0.07%), 40% methanol (0.07%) and 2 hours of reflux extraction was effective in order of 60% methanol (0.21%) > 40% methanol (0.17%) > 80% methanol (0.14%). To compare the extraction efficiency of rubiadin according to the extraction methods and time for high rubiadin content, the extracts of rubiadin in noni adventitious roots were isolated with the methods of ultrasonic extraction, shaking extraction and reflux extraction. Rubiadin extracted from the methods of ultrasonic waves and shaking displayed the highest contents at 8 and 24 hours, respectively.
Keywords
HPLC; methanol; reflux extraction; shaking extraction; ultrasonic extraction;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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