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Molecular Genetics of Brassicaceae Based on AFLP Display  

Kwon, Soo-Jin (National Institute of Agricultural Biotechnology)
Lim, Ki-Byung (National Institute of Agricultural Biotechnology)
Lim, Myung-Ho (National Institute of Agricultural Biotechnology)
Park, Jee-Young (National Institute of Agricultural Biotechnology)
Kim, Jin-A (National Institute of Agricultural Biotechnology)
Kim, Jung-Sun (National Institute of Agricultural Biotechnology)
Lee, Soo-Seong (Biiobreeding)
Park, Beom-Seok (National Institute of Agricultural Biotechnology)
Jin, Yong-Moon (National Institute of Agricultural Biotechnology)
Publication Information
Horticultural Science & Technology / v.25, no.2, 2007 , pp. 75-81 More about this Journal
Abstract
Genetic diversity and relationship in the related plant taxa is a prerequisite for crop improvement. Amplified fragment length polymorphism (AFLP) fingerprinting is one of the powerful and widely used tools, which has found wide spread application in numerous studies ranging from genetic mapping to diversity surveys in plants and other organisms. Genome relationship among diploid and allotetraploid Brassica taxa, and between interspecific hybrid or intergeneric hybrid and their parental species were investigated using AFLP display. The two primer pairs for AFLP reactions and seven EcoRI (+2) and M5eI (+3) primer combinations were used in the measurement of genetic relationships. Genetic similarities among nine accessions were estimated by Nei’s distance equation, and cluster analysis was performed using the unweighed pair group method (UPGMA) in the computer program NTSYS-pc. One intergeneric hybrid between Brassica and Raphanus showed 2n=4x=38 chromosomes, of which Raphanus chromosome segment were translocated with B. rapa chromosome.
Keywords
AFLP fingerprinting; genetic relationship; hybrid;
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