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http://dx.doi.org/10.3796/KSFT.2014.50.4.447

Changes in the growth and biochemical composition of Chaetoceros calcitrans cultures using light-emitting diodes  

An, Heui-Chun (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI)
Bae, Jae-Hyun (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI)
Kwon, O-Nam (East Costal Life Science Institute Gangneung-Wonju National University)
Park, Heum-Gi (Department of Marine Bioscience Gangneung-Wonju National University)
Park, Jin-Chul (Department of Marine Bioscience Gangneung-Wonju National University)
Publication Information
Journal of the Korean Society of Fisheries and Ocean Technology / v.50, no.4, 2014 , pp. 447-454 More about this Journal
Abstract
The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR${\geq_-}$CON>LB${\geq_-}$LW${\geq_-}$LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures ($6.3{\mu}g/mL$), whereas LW showed the lowest ($3.6{\mu}g/mL$; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.
Keywords
Chaetoceros calcitrans; LED; Specific growth rate; Fatty acid; Fucoxanthin;
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