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http://dx.doi.org/10.5656/KSAE.2022.06.0.037

Complete Genome Analysis of Spodoptera exigua Nucleopolyhedrovirus Isolated in Korea  

Jae Bang, Choi (Optipharm Inc.)
Hyun-Soo, Kim (Department of Plant Medicine, College of Agriculture, Life & Environment Science, Chungbuk National University)
Soo-Dong, Woo (Department of Plant Medicine, College of Agriculture, Life & Environment Science, Chungbuk National University)
Publication Information
Korean journal of applied entomology / v.61, no.3, 2022 , pp. 449-460 More about this Journal
Abstract
The morphology and whole genome sequence of Spodoptera exigua nucleopolyhedrovirus K1 (SeNPV-K1) isolated in Korea were analyzed for the use as an eco-friendly control source against S. exigua. The polyhedra of SeNPV-K1 was amorphous with a size of 0.6-1.8 ㎛, and there was no external difference with the previously reported SeNPV. As a result of analyzing the nucleotide sequence of the whole genome, it was composed of 135,756 bp, which is 145 bp more than that of the previously reported SeNPV. The G+CG+C content was 44% and there were 6 homologous repeated sequences, so there was no significant difference from the previous report. As a result of ORF analysis, SeNPV-K1 had 137, two fewer than those previously reported, and 4 ORFs present only in SeNPV-K1 were confirmed. These 4 ORFs are non-essential genes and were not considered to have a significant influence on the characteristics of the SeNPV. The genome vista analysis showed that the overall sequence similarity between SeNPV-K1 and the previously reported SeNPV was very high. The whole genome of SeNPV-K1 analyzed for the first time in Korea was found to be similar to the previously reported SeNPV, but it was confirmed that it was a novel resource in Korea with different isolate.
Keywords
Spodoptera exigua; Nucleopolyhedrovirus; SeNPV-K1; Whole genome; ORF analysis;
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