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http://dx.doi.org/10.5656/KSAE.2012.09.0.037

A PCR Method to Distinguish Matsumuraeses phaseoli from M. falcana Based on the Difference of Nucleotide Sequence in the Mitochondrial Cytochrome c Oxidase Subunit I  

Seo, Bo Yoon (National Institute of Crop Science)
Jung, Jin Kyo (National Institute of Crop Science)
Cho, Jum Rae (National Academy of Agricultural Science)
Kim, Yonggyun (Major in Plant Medicine, School of Bioresource Sciences, Andong National University)
Park, Chang Gyu (National Academy of Agricultural Science)
Publication Information
Korean journal of applied entomology / v.51, no.4, 2012 , pp. 365-370 More about this Journal
Abstract
The two closely related major leguminous crop pests in Korea, Matsumuraeses phaseoli and M. falcana (Lepidoptera: Tortricidae) have very similar morphological characters, which occasionally give rise to a failure in distinguishing between the two. In this study, we report an easy PCR-SSP method to distinguish between them, with a sequence specific primer set (P-SF2, F-SF3, and C-SR3) based on single nucleotide mismatch in 3' terminal base of a primer, which is found in the mitochondrial cytochrome c oxidase subunit I DNA (mtCOI). Through application of this method, each species may be clearly identified in terms of its PCR band size and pattern, only one band (245 bp) for M. falcana and one (409 bp) or two bands (409 bp & 245 bp) for M. phaseoli.
Keywords
Matsumuraeses phaseoli; M. falcana; mitochondrial cytochrome c oxidase subunit I (mtCOI); PCR-SSP;
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Times Cited By KSCI : 4  (Citation Analysis)
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