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http://dx.doi.org/10.5656/KSAE.2011.09.0.56

Optimization for Mycelial Growth and Inhibitory Effect on Nitric Oxide Production of Cordyceps nutans Pat.  

Lee, Ki-Man (College of Pharmacy and Institute of Chronic Disease, Sahmyook University)
Lee, Geum-Seon (College of Pharmacy and Institute of Chronic Disease, Sahmyook University)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Lim, Sung-Cil (College of Pharmacy, Chungbuk National University)
Kang, Tae-Jin (College of Pharmacy and Institute of Chronic Disease, Sahmyook Univer)
Publication Information
Korean journal of applied entomology / v.50, no.4, 2011 , pp. 307-314 More about this Journal
Abstract
Cordyceps (vegetable wasp and plant worm), an entomopathogenic fungi, has been used as a herbal medicine in Asian countries since ancient times. Cordyceps nutans is common but there is little research on this species. This study investigated the optimal culture conditions of C. nutans and the inhibitory effect on nitric oxide (NO) production in RAW 264.7 cell treated culture broth. The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 7.0-8.0. Mycelial growth was highest on mushroom complete medium (MCM), V8 juice agar (V8A), and yeast malt dextrose (YMD) medium. Mycelial growth on mushroom minimal medium (MMM) did not occur, so nutrient source was essential. Dextrose and sucrose as carbon sources, and ammonium citrate as a nitrogen source were satisfactory for mycelial growth. Cytotoxicity of C. nutans culture broth was not found in RAW 264.7 cells. C. nutans culture broth suppressed NO production of lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. Thus, our results provided the optimal conditions for cultivation of C. nutans and showed that C. nutans may have excellent physiological activities.
Keywords
Mycelial growth; Cordyceps nutans; Cultivation; Cytotoxicity; Nitric oxide; NO;
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