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http://dx.doi.org/10.5656/KSAE.2009.48.3.377

Total Phenol Contents and DPPH Radical Scavenging Activity of Entomopathogenic Fungi  

Lee, Ki-Man (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Song, Ha-Suk (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yeo, Joo-Hong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, Kwang-Gill (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Bae, Yoon-Hwan (Department of Life Science, College of Natural Sciences, Daejin University)
Publication Information
Korean journal of applied entomology / v.48, no.3, 2009 , pp. 377-383 More about this Journal
Abstract
This study was conducted to investigate the total phenol contents, antioxidative activities and antibacterial activities of twenty species of entomopathogenic fungi. The total phenol content was highest in Aspergillus flavus ($553.0{\pm}52.15{\mu}g/g$) and A. parasiticus ($529.9{\pm}60.10{\mu}g/g$). On the other hand those in other strains were within the range of $26.6{\sim}121.9{\mu}g/g$. The antioxdative activity was shown in the most of strains and the highest DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging activity was observed in A. flavus ($90.9{\pm}2.90%$) and A. parasiticus ($77.9{\pm}4.13%$). This result indicated that the antioxidative activities were very correlated with the total phenol contents. The antibacterial activitiy was found in the every tested pathogenic bacteria. Especially, the antibacterial activity was strongest against Listeria monocytogenes and Escherchia coli.
Keywords
Entomopathogenic fungi; Total phenol contents; Antioxidative activities; DPPH; Antibacterial activities;
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1 Kitahara, K, Y. Matsumoto, H. Veda and R. Veoka. 1992, A remarkable antioxidation effect of natural phenol derivatives on the autoxidation of y-irradiated methyl linoleate. Chem. Phann. Bull. 40: 2208-2209   DOI
2 Lee, G.D., H.G. Chang and H.K Kim. 1997, Antioxidative and nitrite-scavenging activities of edible mushrooms, Kor. J. Food Sci. Technol. 29: 432-436
3 Lee, Y.S., EJ. Joo and N.W. Kim. 2006, Polyphenol contents and antioxidant activity of Lepista nuda. 1. Kor. Soc. Food Sci. Nutr. 35:1309-1314   DOI   ScienceOn
4 Whang, H.J., W.S. Han and K.R. Yoon. 2001, Quantitative analysis of total phenolic content in apple, Analytical Sci.Technol. 14: 377-383
5 Konyahoglu, S., H. Saglam and B. Kivcak. 2005, a-tocopherol, flavonoid, and phenol contents and antioxidant activity of Ficus carica leaves, Pham. BioI. 43: 683-686
6 Lee, K.M., LP. Hong, S.H. Nam, G.B. Sung and Y.H. Bac. 2008, The cultural characteristics and antibacterial activities of Cordyceps militaris and Paecilomyces tenuipes, Kor. J. Appl. Entomol. 47:479-486   DOI   ScienceOn
7 Nam, S.H., H.J. Yoon and S.E. Kim. 2003,. Morphological characteristics of Aspergillus jlavus link causing stonebrood disease in Korean native bumblebee, Bombus ignitus. Kor. J.Apicul. 18: 43-48
8 Shih, I.L., K.L. Tsai and C. Hsieh. 2007, Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris, Biochem.Eng. J. 33: 193-201   DOI   ScienceOn
9 Lee, J.H. and S.R. Lee. 1994, Analysis of phenolic substances content in Korean plant foods, Kor. J. Food Sci. Technol. 26: 310-316
10 Lee, H.O., K.H. Lee, N.K Park, S.I. Jeong, S.H. Baek and D.M. Han. 2000, Antibacterial effects of Sophora jlavescens on Streptococcus mutans, Kor. J. Food & Nutr. 13: 539-546
11 Chae, s., 1.S. Kim, KA. Kang, H.D. Bu, Y. Lee, l.W. Hyun and S.S. Kang. 2004, Antioxidant activity of jionoside D from Clerodendron trichotomum, BioI. Pharm. BulL 27: 1504-1508   DOI   ScienceOn
12 Moon, Y.G. and M.S. Heo. 2007, Screening of antioxidative and antibacerial activity from methanol extracts of indigenous plants, Jeju-island. Kor. J. Biotechnol. Bioeng. 22: 78-83
13 Zhu, J.S., G.M. Halpern and K. Jones. 1998, The scientific rediscovery of an ancient Chines herbal medicine: Cordyceps sinensis, Part I, J. Altern. Complement. Med. 4: 289-303   DOI   ScienceOn
14 Shah, P.A. and J.K. Pel!. 2003, Entomopathogenic fungi as biological control agents. Appl. Microbiol. Biotechnol. 61:413-423   DOI   PUBMED   ScienceOn
15 Wiseman, D.W and E.H. Marth. 1981, Growth and aflatoxin production by Aspergillus parasiticus when in the presence of Streptococcus lactis, Mycopathologia. 73: 49-56   DOI   ScienceOn
16 Kang, S.J., J.S. Kang and KH. Chung. 2001, The effect of mixed culture with Aspergillus jlavuv, Aspergilus niger and Penicillium griseofillvum on aflatoxin and patulin production, J. Fd Hyg. Safety 16:206-211
17 Martinez, S., L. Valek, J. Piljac and M. Metikos-Hukovic. 2005, Dctermination of wine antioxidant capacity by derivative potentiometric titration with electrogenerated chlorine, Eur. Food Res. Technol 220: 658-661   DOI   ScienceOn
18 Woo, W.S. 1996, Methods in studies of natural chemistry, 61-63 pp. SNU press. Korea.
19 Shim, J.Y., Y.S. Lee, S.S. Lim, K.H. Shin, J.E. Hyun, S.Y. Kim and E.B. Lee. 2000, Pharmacological activities of Paecilomyces japonica, A new type Cordyceps sp, Kor. J. Pharmacogn. 31:163-167
20 Jang, S.H., E.A. Yu, KS. Han, S.C. Shin, H.K. Kim and S.G. Lee. 2008, Changes in total polyphenol contents and DPPH radical scavenging activity of Agrimonia pi/osa according to harvest time and various part, Kor. J. Medicinal Crop Sci. 16:397-40l
21 Kuo, Y.C., c.Y. Lin, W.J. Tsai, C. L. Wu, C.F. Chen and M.S. Shiao. 1994, Growth inhihitors against tumor cells in Cordyceps sinensis other than codycepin and polysacharides, Cancer Invest12: 611-615   DOI   ScienceOn