Browse > Article
http://dx.doi.org/10.5656/KSAE.2007.46.2.319

Determination of the Optimal Concentration of Fetal Bovine Serum for the Growth of Two Insect Cell and Viruses  

Lee, Jae-Kyung (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University)
Koo, Hyun-Na (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University)
Woo, Soo-Dong (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University)
Publication Information
Korean journal of applied entomology / v.46, no.2, 2007 , pp. 319-324 More about this Journal
Abstract
To determine the optimal concentration of fetal bovine serum (FBS) on the growth of insect cells and the multiplicity of viruses, the growth of cells (Sf21 and Bm5) and viruses were examined on the various concentrations of FBS. In view of the viability, growth speed, proliferation of cells and the amount of FBS, the most proper concentration for the cell culture were 7% and 5% for Sf21 and Bm5, respectively. The multiplicity of viruses at the various concentrations of FBS was similar in both cell lines at 5 days post-infection (p.i.). However, it differed significantly at 2 and 3 days p.i. The proper concentration of FBS were 10% and 3% for Sf21 at 2 and 3 days p.i., respectively, and 5% for Bm5 at both 2 and 3 days p.i. These results suggested that the optimal concentration of FBS should be determined according to the used cell lines and viruses for their optimum production.
Keywords
Insect cell; Nucleopolyhedrovirus; Fetal bovine serum(FBS); Cell growth; Virus multiplicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Maeda, S., T. Kawai, M. Obinata, H. Fujiwara, T. Horiuchi, Y. Saeki, Y. Soto and M. Furusawa. 1985. Production of human a-interferon in silkworm using a baculovirus vector. Nature 315: 592-594   DOI   ScienceOn
2 Smith, G.E., M.D. Summers and M.J. Fraser. 1983. Production of human ${\beta}$-interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3: 2156-2165   DOI
3 Taticek, R.A., C. Choi, S.E. Phan, L.A. Palomares and M.L. Shuler. 2001. Comparison of growth and recombinant protein expression in two different insect cell lines in attached and suspension culture. Biotechnol. Prog. 17: 676-684   DOI   ScienceOn
4 Wickham, T.J., R.R. Granados, H.A. Wood, D.A. Hammer and M.L. Shuler. 1990. General analysis of receptor-mediated viral attachment to cell surfaces. Biophys. J. 58: 1501-1516   DOI   ScienceOn
5 Yunker, C.E., J.L. Vaughn and J. Cory. 1967. Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph. Science, 155: 1565-1566   DOI   ScienceOn
6 Petricevich, V.L., L.A. Palomares, M. Gonzalez and O.T. Ramirez. 2001. Parameters that determine virus adsorption kinetics: toward the design of better infection strategies for the insect cellbaculovirus expression system. Enzyme Microb. Technol. 29: 52-61   DOI   ScienceOn
7 Hong, H.K., S.D. Woo, J.Y. Choi, H.K. Lee, M.H. Kim, Y.H. Je, and S.K. Kang. 2000. Characterization of four isolates of Bombyx mori nucleopolyhedrovirus. Arch. Virol. 145: 2351-2361   DOI   ScienceOn
8 Joshi, L., T.R. Davis, T.S. Mattu, P.M. Rudd, R.A. Dwek, M.L. Shuler, M. L. and H.A. Wood. 2000. Influence of baculovirushost cell interactions on complex N-linked glycosylation of a recombinant human protein. Biotechnol. Prog. 16: 650-656   DOI   ScienceOn
9 Zhang, J., N. Kalogerakis, L.A. Behie and K. Iatrou. 1992. Investigation of reduced serum and serum-free media for the cultivation of insect cells (Bm5) and the production of baculovirus (BmNPV). Biotechnol. Bioeng. 40: 1165-1172   DOI
10 King, G., J. Kuzio, A. Daugulis, P. Faulkner, B. Allen, J. Wu and M. Goosen. 1991. Assessment of virus production and chloramphenicol acetyl transferase expression by insect cells in serum-free and serum-supplemented media using a temperature sensitive baculovirus. Biotechnol. Bioeng. 38: 1091-1099   DOI
11 Power, J.F., S. Reid, P.F. Greenfield and L.K. Nielsen. 1996. The kinetics of baculo virus adsorption to insect cells in suspension culture. Cytotechnology, 21: 155-163   DOI   ScienceOn
12 O'Reilly, D.R., L.K. Miller and V.A. Luckow. 1992. Baculovirus expression vectors: a laboratory manual. 347 pp. Freeman, New York
13 Goodwin, R.H. 1975. Insect cell culture: improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro, 11: 369-378   DOI
14 Broussard, D.R. and M.D. Summers. 1989. Effects of serum concentration and media composition on the level of polyhedrin and foreign gene expression by baculovirus vectors. J. Invertebr. Pathol. 54: 144-150   DOI
15 Ikonomou, L., Y.J. Schneider and S.N. Agathos. 2003. Insect cell culture for industrial production of recombinant proteins. Appl. Microbiol. Biotechnol. 62: 1-20   DOI
16 Nielsen, L.K. 2000. Virus production from cell culture, kinetics. pp 1217-1230. In The encyclopedia of cell technology, ed. by R.E. Spier. Wiley, New York
17 Taticek, R.A. and M.L. Shuler. 1997. Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system. Biotechnol. Bioeng. 54: 142-152   DOI   ScienceOn