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Occurrence of Plant Parasitic Nematodes in Chrysanthemum and ITS and D3-28S rDNA Characterization of Pratylenchus spp.  

Han, Hye-Rim (Division of Forest Diseases and Insect pests, Korea Forest Research Institute)
Lee, Jae-Kook (Applied Entomology Division, National Institute of Agricultural Science & Technology, RDA)
Choi, Dong-Ro (Division of Forest Diseases and Insect pests, Korea Forest Research Institute)
Han, Man-Jong (Division of Forest Diseases and Insect pests, Korea Forest Research Institute)
Park, Byeong-Yong (Ginseng & Medicinal Crop Division, National Institute of Crop Science, RDA)
Publication Information
Korean journal of applied entomology / v.45, no.3, 2006 , pp. 293-299 More about this Journal
Abstract
A survey was conducted to find out the major plant parasitic nematode in Chrysanthemum morifolium fields in Korea from May to June in 2005. A genus of Pratylenchus was determined as the most important plant parasitic nematode based on analysis of total 50 samples from 8 cities of chrysanthemum field. Pratylenchus showed 86% occurrence rate and average numbered 1,095 per 200cc soils and 1g root. Five Pratylenchus isolates, 'Muan', 'Masan', 'Tean', 'Gumi', 'Jeongup', were selected for the molecular identification of the species of Pratylenchus, and ITS and D3-28S ribosomal DNA were amplified by PCR. For the ITS, only 'Muan' isolate was differentiated by total 1 kb PCR amplification, which was 200 bp larger than all the other isolates. There was no size variation in amplified D3-28S rDNA and all isolate represented approximately 320 bp of PCR product. Sequence data of D3-28S rDNA were analysed by MegAlign program in DNASTAR software and phylogenetic tree was constructed. Sequence homology was 100% between 'Gumi' isolate and 'Tean' isolate and 'Jeongup' isolate was also close to these isolates by 99.7% sequence homology. 'Gumi', 'Tean' group and 'Jeongup' isolate were determined to be closely related to Pratylenchus vulnus by 96.7% and 96.3% similarity in respectively. D3 sequence of 'Masan' isolate was 100% identical to P. penetrans, and 'Muan' isolate showed 99.7% similarity to P. brachyurus. This result was congruent with the branch divergence pattern shown in phylogenetic tree.
Keywords
Pratylenchus; ITS; D3-28S; Molecular identification; Chrysanthemum morifolium;
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