Browse > Article

Inhibitory Effect of Cotesia plutellae Bracovirus (CpBV) on Development of a Non-natural Host, Spodoptera exigua  

Kim Yonggyun (School of Bioresource Sciences, Andong National University)
Kim Jiwon (School of Bioresource Sciences, Andong National University)
Publication Information
Korean journal of applied entomology / v.43, no.3, 2004 , pp. 217-223 More about this Journal
Abstract
Polydnavirus is a symbiotic virus of some endoparasitic wasps and plays crucial roles in inhibiting immune responses and retarding development of the parasitized hosts. Cotesia plutellae bracovirus (CpBV) is a polydnavirus suggesting a major causative to change developmental physiology of the parasitized host. Here, we investigated whether CpBV can interrupt development of non-natural host. Beet armyworm, Spodoptera exigua, is used as a non-permissible host for parasitization of C. plutellae. Extract from the calyx region of C. plutellae contained CpBV, which was confirmed by immunoblotting with a polyclonal antibody raised against CpBV. One female equivalent of CpBV extract was injected into hemocoel of late 4th instar larvae of S. exigua. The injected larvae showed delayed larval period, decrease of body weight gain, and inability of pupal metamorphosis. These inhibitory effect of the CpBV extract was rescued by injection along with CpBV antibody, though the antibody itself did not give any effect on development of the larvae. This result clearly shows that CpBV can interrupt developmental physiology of a non-natural host for its symbiotic wasp.
Keywords
Cotesia plutellae; Metamorphosis; Parasitization; Polydnavirus; Spodoptera exigua;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Asgari, S., O. Schmidt and U. Theopold. 1997. A polydnavirus encoded protein of an endoparasitoid is an immune suppressor J. Gen. Virol. 78: 3061-3070
2 Beckage, N.E., F.F. Tan, K.W. Schleifer, R.D. Lane and L.L. Cherubin. 1994. Characterization and biological effects of Cotesia congregata polydnavirus on host larvae of the tobacco hornworm, Manduca sexta. Arch. Insect Biochem. Physiol. 26: 165-195   DOI   ScienceOn
3 Glatz, R., O. Schmidt and S. Asgari. 2003. Characterization of a novel protein with homology to C-type lectin expressed by the Cotesia rubecula bracovirus in larvae of the lepidopteran host, Pieris rapae. J. Biol. Chem. 278: 19743-19750   DOI   ScienceOn
4 Li, S., P. Falabella, I. Kuriachan, S.B. Vinson, D.W. Borst, C. Malva and F. Pennacchio. 2003. Juvenile hormone synthesis, metabolism, and resulting haemolymph titer in Heliothis virescens larvae parasitized by Toxoneuron nigriceps. J. Insect Physiol. 49: 1021-1030   DOI   ScienceOn
5 Nakamatsu, Y., Y. Gyotoku and T. Tanaka. 2001. The endoparasitoid Cotesia kariyai (Ck) regulates the growth and metabolic efficiency of Pseudaletia separata larvae by venom and Ck polydnavirus. J. Insect Physiol. 47: 573-584   DOI   ScienceOn
6 Stoltz, D.B. and S.B. Vinson and E.A. Mackinnon 1976. Baculovirus-like particles in the reproductive tract of female parasitoid wasps. Can. J. Microbiol. 22: 1013-1023   DOI   ScienceOn
7 Bae, S. and Y. Kim. 2004. Host physiological changes due to parasitism of a braconid wasp, Cotesia plutellae, on diamondback moth, Plutella xylostella. Comp. Biochem. Physiol. 138A: 39-44   DOI   ScienceOn
8 Vass, E., A.J. Nappi and Y. Carton. 1993. Comparative study of immune competence and host susceptibility in Drosophila melanogaster parasitized by Leptopilina boulardi and Asobara tabida. J. Parasitol. 79: 106-112   DOI   ScienceOn
9 Oh, M.R., S.S. Kim, J.D. Park, J.C. Paik and D.I. Kim. 1997. Biological characteristics of Cotesia plutellae (Hymenoptera: Braconidae), a larval parasitoid of Plutella xylostella (Leipdoptera: Yponomeutidae). Korean J. Entomol. 27: 79-84
10 Stoltz, D.B. and S.B. Vinson. 1979. Viruses and parasitism in insects. Adv. Virus Res. 24: 125-171   DOI   PUBMED
11 Kim, Y. and B.A. Webb. 2003. Effect of a cys-motif protein, VHv1.4, of Campoletis sonorensis Ichnovirus on the translation of lysozyme mRNA. J. Asia-Pacific Entomol. 6: 243-246   DOI   ScienceOn
12 Pennacchio, F., P. Falabella, R. Sordetti, P. Varricchio, C. Malva and S.B. Vinson. 1998. Prothoracic gland inactivation in Heliothis virescens (F.) (Lepidoptera: Noctuidae) larvae parasitized by Cardiochiles nigriceps Viereck (Hymenoptera: Braconidae). J. Insect Physiol. 44: 845-857   DOI   ScienceOn
13 Webb, B.A. 1998. Polydnavirus biology, genome structure, and evolution. pp. 105-139. In The insect viruses, eds. by L.K. Miller and L.A. Ball. Plenum Press, New York
14 Federici, B.A., J.J. Hamm and E.L. Stryer. 1991. Ascoviridae. pp. 339-349. In Atlas of invertebrate viruses, eds. by J.R. Adams and J.R. Bonam. CRC Press, Boca Raton, FL
15 Schmidt, O, S. Asgari, M. Beck and U. Theopold. 2000. Host defence manipulation by parasitoid wasps and the problem of assessing host specificity. pp. 29-37. In Hymenoptera: evolution, biodiversity and biological control, eds. by A.D. Austin and M. Dowton. CSIRO Publishing, Ausralia
16 Bigot, Y., A. Rabouille, P.-Y. Sizaret, M.-H. Hamelin and G. Periquet. 1997. Particle and genomic characteristics of a new member of the Ascoviridae: Diadromus pulchellus ascovirus. J. Gen. Virol. 78: 1139-1147
17 Carton, Y. and A.J. Nappi. 1991. The Drosophila immune reaction and the parasitoid capacity to evade it: genetic and coevolutionary aspect. Acta Oecol. 12: 89-104
18 Moon, J. and Y. Kim. 2003. Purification and characterization of vitellin and vitellogenin of the beet armyworm, Spodoptera exigua (Noctuidae: Lepidoptera). J. Asia-Pacific Entomol. 6: 37-43   DOI   ScienceOn
19 Webb, B.A. and S. Luckhart. 1994. Evidence for an early immunosuppressive role for related Campoletis sonorensis venom and ovarian proteins in Heliothis virescens. Arch. Insect Biochem. Physiol. 26: 147-163   DOI   ScienceOn
20 Cui, L., A.I. Soldevila and B.A. Webb. 2000. Relationships between polydnavirus gene expression and host range of the parasitoid wasp Campoletis sonorensis. J. Insect Physiol. 46: 1397-1407   DOI   ScienceOn
21 Kim, D., Y.I. Lee and Y. Kim. 2004. Juvenile hormone action on vitellogenesis of the beet armyworm, Spodoptera exigua. J. Asia-Pacific Entomol. 7: 73-79   DOI   ScienceOn
22 Shelby, K. and B.A. Webb. 1997. Polydnavirus infection inhibits translation of specific growth-associated host proteins. Insect Biochem. Mol. BioI. 27: 263-270   DOI   ScienceOn
23 Gho, H.K., S.G. Lee, B.P. Lee, K.M. Choi and J.H. Kim. 1991. Simple mass-rearing of beet armyworm, Spodoptera exigua (H$\"u$bner) (Lepidoptera: Noctuidae), on an artificial diet. Korean J. Appl. Entomol. 29: 180-183
24 Webb, B.A. and M.D. Summers. 1992. Stimulation of polydna-virus replication by 20-hydroxyecdysone. Experientia 48: 1018-1022   DOI   PUBMED   ScienceOn
25 Cui, L., A.I. Soldevila and B.A. Webb. 1997. Expression and hemocyte-targeting of a Campoletis sonorensis polydnavirus cysteine-rich gene in Heliothis virescens larvae. Arch. Insect Biochem. Physiol. 36: 251-271   DOI   ScienceOn
26 Lovallo, N., B.A. McPheron and D.L. Cox-Foster. 2002. Effects of the polydnavirus of Cotesia congregata on the immune system and development of non-habitual hosts of the parasitoid. J. Insect Physiol. 48: 517-526   DOI   ScienceOn
27 Lee, S. and Y. Kim. 2004. Alteration of endocrine signals in late larval stages of diamondback moth, Plutella xylostella, by parasitization of an endoparasitic wasp, Cotesia plutellae. J. Asia-Pacific Entomol. (submitted)
28 Krell, P.J., M.D. Summers and S.B. Vinson. 1982. Virus with a multipartite superhelical DNA genome from the ichneumonid parasitoid Campoletis sonorensis. J. Virol. 43: 859-870
29 Stoltz, D.B. 1993. The polydnavirus life cycle. pp. 167-187. In Parasites and pathogens of insects. Volume 1: Parasites, eds. by N.E. Beckage, S.N. Thompson and B.A. Federici. Academic Press, New York
30 SAS Institute, 1989. SAS/STAT user's guide, Release 6.03, Ed. Cary, N.C.
31 Webb, B.A., N.E. Beckage, Y. Hayakawa, P.J. Krell, B. Lanzrein, D.B. Stoltz, M.R. Strand and M.D. Summers. 2000. Polydnavirus. pp. 253-260. In Virus taxonomy, eds. by M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D.J. McGeoch, C.R. Pringle and R.B. Wickner. Academic Press, New York