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http://dx.doi.org/10.6114/jkood.2022.35.4.063

Skin Moisturizing Activity of Unripe Apple(Immature Fruit of Malus pumila) in Mice  

Park, Hye Rim (Dept. of Anatomy and Histology, College of Korean Medicine, Daegu Haany University)
Kim, Jae Kwang (Korean Medicine-Application Center, Korea Institute of Oriental Medicine)
Lee, Jae Kyoung (CNS Pharm Korea Co. Ltd.)
Choi, Beom Rak (Nutracore Co. Ltd.)
Kim, Jong Dae (Dept. of Pulmonary Internal Medicine, Daegu Haany University Daegu Oriental Hospital, Daegu Haany University)
Ku, Sae Kwang (Dept. of Anatomy and Histology, College of Korean Medicine, Daegu Haany University)
Jegal, Kyung Hwan (Dept. of Korean Medical Classics, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.35, no.4, 2022 , pp. 63-74 More about this Journal
Abstract
Objectives : Skin aging is generally characterized by wrinkles, sagging, loss of elasticity roughness, pigmentation and dryness. This changes is caused by reducing the elements constituting the extracellular matrix contributing to the physiological properties of the skin, such as collagen fiber, elastic fiber, and hyaluronic acid. Adequate skin hydration is important to maintain normal skin function and reduce skin aging. The present study is objective to observe skin moisturizing effects of Unripe apple(UA, Immature fruit of Malus pumila Mill) in vivo and its underlying molecular mechanisms. Methods : ICR mice were orally administerd UA(100, 200 and 400mg/kg/day) for 8 weeks, and skin water contents and the expression of transforming growth factor (TGF)-𝛽1, ceramide, hyaluronan and collagen type I(COL1) were measured in dorsal back skin of the mice. Gene expression of hyaluronan synthase(HAS1, HAS2, HAS3), collagen synthase(COL1A1, COL1A2) and TGF-𝛽1 were also determined by realtime RT-PCR. Results : Skin water contents and the expression of TGF-𝛽1, ceramide, COL1 and hyaluronan were significantly increased in UA group(100, 200 and 400mg/kg/day) compared to vehicle control. The mRNA expression of HAS isoform(HAS1, HAS2, HAS3), COL1A1, COL1A2, and TGF-𝛽1 were also significantly increased by UA. Conclusions : UA has skin moisturizing effects and enhancement activities in skin function related components(COL1, hyaluronan, ceramide and TGF-𝛽1). These results suggested that UA can be a developing candidate for developing alternative skin protective agent or functional food ingredient.
Keywords
Unripe apple; Skin hydration; Skin moisturizing; Skin aging;
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1 Uitto J, Bernstein EF. Molecular mechanisms of cutaneous aging: connective tissue alterations in the dermis. J Investig Dermatol Symp Proc. 1998;3(1):41-4.
2 Ahmed IA, Mikail MA, Zamakshshari N, Abdullah AH. Natural anti-aging skincare: role and potential. Biogerontology. 2020;21(3):293-310.   DOI
3 Burda S, Oleszek W, Lee CY. Phenolic compounds and their changes in apples during maturation and cold storage. J of Agric Food Chem. 1990;38(4):945-8.   DOI
4 Lee JA. Physicochemical Character and Antioxidant Activity of Unripened Apples in Korea. Cul Sci & Hos Res. 2018;24(8):30-7.
5 Ahmed IA, Mikail MA, Zamakshshari NH, Mustafa MR, Hashim NM, Othman R. Trends and challenges in phytotherapy and phytocosmetics for skin aging. Saudi J Biol Sci. 2022;29(8):103363.   DOI
6 Itano N, Sawai T, Yoshida M, Lenas P, Yamada Y, Imagawa M, et al. Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties. J Biol Chem. 1999;274:25085-92.   DOI
7 Mauviel A. Transforming growth factor-beta signaling in skin: stromal to epithelial cross-talk. J Invest Dermatol. 2009;129(1):7-9.   DOI
8 Rock K, Grandoch M, Majora M, Krutmann J, Fischer JW. Collagen fragments inhibit hyaluronan synthesis in skin fibroblasts in response to ultraviolet B (UVB): new insights into mechanisms of matrix remodeling. J Biol Chem. 2011;286:18268-76.   DOI
9 Uitto J. Understanding premature skin aging. N Engl J Med. 1997;337(20):1463-5.   DOI
10 Zhang S, Duan E. Fighting against skin aging: The way from bench to bedside. Cell Transplant. 2018;27(5):729-38.   DOI
11 Verdier-Sevrain S, Bonte F. Skin hydration: a review on its molecular mechanisms. J Cosmet Dermatol. 2007;6(2):75-82.   DOI
12 Osada K, Ogino Y, Nakamura S. Effect of dietary apple polyphenol on metabolic disorder of lipid in rats given oxidized cholesterol. Proc Jpn Conf Biochem Lipids. 1997;39:317-20.
13 Michalak M, Pierzak M, Krecisz B, Suliga E. Bioactive compounds for skin health: a review. Nutrients. 2021;13(1):203.   DOI
14 Kim JD, Koh BH. The Bibiographical investigation of the apple and Neung-keum. J Kor Med. 1998;19(1):339-48.
15 Korean Interllectual Property Office, Korean Institute of Oriental Medicine. Pyeonggwa(苹果) [Internet]. 2007. Available from: URL: https://doi.org/10.20929/KTKP.MED.0000083143   DOI
16 Kojima T, Akiyama H, Sasai M, Taniuchi S, Goda Y, Toyoda M, et al. Anti-allergic effect of apple polyphenol on patients with atopic dermatitis: A pilot study, Allergol Int. 2000;49(1):69-73.   DOI
17 Choi JH, Chung SK. Antioxidant and antimicrobial activities of polyphenols isolated from unripe apples (Malus pumila cv. Hongro). Korean J Food Preserv. 2019;26:690-6.   DOI
18 Akiyama H, Sakushima J, Taniuchi S, Kanda T, Yanagida A, Kojima T, et al. Antiallergic effect of apple polyphenols on the allergic model mouse. Biol Pharm Bull. 2000;23(11):1370-3.   DOI
19 Coderch L, Lopez O, De La Maza A, Parra JL. Ceramides and skin function. Am J Clin Dermatol. 2003;4(2):107-29.   DOI
20 Dominik IM, Eileen JA, Marc HE, Remo CA, Eliane WA, Hugo ZI. Activation of TGF-β : a gateway to skin rejuvenation. H&PC Today. 2015;10:1-6.
21 Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: a key molecule in skin aging. Dermatoendocrinol. 2012;4(3):253-8.   DOI
22 Sun Q, Wu J, Qian G, Cheng H. Effectiveness of dietary supplement for skin moisturizing in healthy adults: a systematic review and meta-analysis of randomized controlled trials. Front Nutr. 2022;9:895192.   DOI
23 Kono T, Miyachi Y, Kawashima M. Clinical significance of the water retention and barrier function-improving capabilities of ceramide-containing formulations: a qualitative review. J Dermatol. 2021;48(12):1807-16.   DOI
24 Jacobson A, Brinck J, Briskin MJ, Spicer AP, Heldin P. Expression of human hyaluronan synthases in response to external stimuli. Biochem J. 2000;348:29-35.   DOI
25 Stuhlmeier KM, Pollaschek C. Differential effect of transforming growth factor beta (TGF-beta) on the genes encoding hyaluronan synthases and utilization of the p38 MAPK pathway in TGF-beta-induced hyaluronan synthase 1 activation. J Biol Chem. 2004;279(10):8753-60.   DOI