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http://dx.doi.org/10.6114/jkood.2021.34.3.001

Anti-inflammatory Effect of Geumeunwha-san Water Extract on LPS-induced Raw 264.7 Cells  

Kim, Yeon-Soo (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Kim, Su-Jin (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Jee, Seon-Young (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Hwangbo, Min (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.34, no.3, 2021 , pp. 1-12 More about this Journal
Abstract
Objectives : The purpose of this study is to investigate the anti-inflammatory effect of Geumeunwha-san(GEHS) water extract in vitro. Methods : To evaluate the anti-inflammatory effect of GEHS, Raw 264.7 cells were pretreated with 10-300㎍/㎖ of GEHS for 1hr, and then exposed to 1㎍/㎖ of LPS. MTT assay was used to detect the cell viability. Productions of pro-inflammatory cytokines and NO were measured in culture media. By using immunoblot analysis, protein levels of iNOS and NF-𝜅B were determined. Results : In vitro study, cell viability assay on GEHS treatment of 10-300㎍/㎖ has no cytotoxicity in Raw 264.7 cells. Pretreated 100, 300㎍/㎖ of GEHS had significantly inhibited LPS-induced NO production. And also pretreatment of 100, 300㎍/㎖ GEHS had significantly decreased production of interleukin-6, -1𝛽 and tumor necrosis factor-𝛼 in LPS-activated Raw 264.7 cells. In addition, GEHS reduced LPS-mediated iNOS expression. Moreover I-𝜅B𝛼 expression was significantly induced by GEHS and NF-𝜅B expression was reduced by GEHS. Conclusions : These results suggest the clinical basis of GEHS for the treatment of inflammatory diseases.
Keywords
Geumeunwha-san(GEHS); Anti-inflammation; NF-${\kappa}B$;
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