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http://dx.doi.org/10.6114/jkood.2020.33.2.100

Anti-inflammatory mechanism and Anti-oxidant Effects of Naesohwangryun-tang in LPS-Stimulated RAW 264.7 Macrophage Cells  

Jeon, Seon-Hong (Dept. Of Cosmetic Sciences, Semyung University)
Kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Yong-Min (Dept. Of Cosmetic Sciences, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.33, no.2, 2020 , pp. 100-111 More about this Journal
Abstract
Objectives : The aim of experiment is to examine anti-inflammatory effect and anti-oxidant effect of Naesohwangryun-tang (NSHRT) in LPS-stimulated RAW264.7 macrophage cells. Methods : In the present study, The cell viability was performed by MTT assay. Nitric oxide (NO) production and prostaglandin E2 (PGE2) synthesis were performed by NO assay and ELISA KIT. The anti-oxidant effect was performed by DPPH and ABTS radical scavenging activity. The inhibitory effects of pro-inflammatory mediators and cytokines were confirmed by realtime PCR and western blotting. Results : NSHRT was no cytotoxicity at treated group. NO and PGE2 production were inhibited compared to the LPS treated group and also mRNA and protein expressions were significantly decreased compared to the LPS treated group. Conclusions : According to the above experiments, we confirmed that NSHRT has anti-inflammatory and anti-oxidant effects. It is suggested that NSHRT is potential ingredient of skin diseases.
Keywords
Naesohwangryun-tang (NSHRT); Anti-inflammatory; Anti-oxidant; NO; COX-2;
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1 Coleman JW. Nitric oxide in immunity and inflammation. International Immunopharmacology. 2001;1(8):1397-406.   DOI
2 Masferrer JL, Zweifel BS, Manning PT, Hauser SD, Leahy KM, Smith WG, et al. Selective inhibition of inducible cyclooxygenase 2 in vivo is antiinflammatory and nonulcerogenic. PNAS. 1994;91(8):3228-32.   DOI
3 Kevin A, Bockerstett, Richard JD. Regulation of gastric carcinogenesis by inflammatory cytokines. Cellular and Molecular Gastroenterology and Hepatology. 2017;4(1):47-53.   DOI
4 Brennan FM, Maini RN, Feldmann M. Role of pro-inflammatory cytokines in rheumatoid arthritis. Springer Seminars in Immunopathology. 1998;20(1):133-47.   DOI
5 Dinarello CA. Biologic Basis for Interleukin-1 in Disease. The Journal of The American Society of Hematology. 1996;87(6):2095-147.
6 Kishimoto T, Akira S, Narazaki M, Taga T. Interleukin-6 Family of Cytokines and gp130. The Journal of The American Society of Hematology. 1995;86(4):1243-54.
7 Kim DY. Anti-allergic Effect of Sapindus Mukorossi Fruits Extract in Human Mast Cells. Journal of Investigative Cosmetology. 2019;15(4):389-95.
8 Lawrence T, Fong C. The resolution of inflammation: Anti-inflammatory roles for $NF-{\kappa}B$. The International Journal of Biochemistry Cell Biology. 2010;42(4):519-23.   DOI
9 Kim HK. Role of ERK/MAPK signalling pathway in anti-inflammatory effects of Ecklonia cava in activated human mast cell line-1 cells. Asian Pacific Journal of Tropical Medicine. 2014;7(9):703-8.   DOI
10 Han MH, Lee MH, Hong SH, Choi YH, Moon JS, Song MK, et al. Comparison of Anti-inflammatory Activities among Ethanol Extracts of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus, and their Mixtures in RAW 246.7 Murine Macrophages. Journal of Life Science. 2014;24(3):329-35.   DOI
11 Joseph JB, Yanhua BI, Clifford JB. The Role of p38 Mitogen-Activated Protein Kinase in IL-$1{\beta}$ Transcription. The Journal of Immunology. 1999;162(9):5367-73.
12 Smith JA, Das A, Ray SK, Banik NL. Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases. Brain Research Bulletin. 2011;87(1):10-20.   DOI
13 Hori M. Kita M, Torihashi S, Miyamoto S, Won KJ, Sato K, et al. Upregulation of iNOS by COX-2 in muscularis resident macrophage of rat intestine stimulated with LPS. Journal of Physiology Gastrointestinal and Liver Physiology. 2001;280(5):930-8.   DOI
14 Samuel JA, Keith, Elazibeth AM. Specific Inhibitors of p38 and Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase Pathways Block Inducible Nitric Oxide Synthase and Tumor Necrosis Factor Accumulation in Murin Macrophages Stimulated with Lipopolysaccharide and Interferon-${\gamma}$. The journal of Infectious Diseases. 1999;179(4);939-44.   DOI
15 Heo J. Donguibogam. Hadong:Donguibogam Publisher. 2006:1539-86.
16 Ahn JH, Sim SY, Kim KJ. The Anti-Inflammatory Effects of NeiShu-HuangLianTang In Experi-mental Animals. Journal Korean Med Ophthalmol Otolaryngol Dermatol. 2004;17(3):8-17.
17 Ko WS, Kim KJ, Kim NK, Kim YB, Kim JH, Kim HT, et al. Text of Traditional Korean Dermatology & Surgery. Busan:Seonu. 2007:139-62.
18 Chun SC, Jee SY, Lee SK. The Antimicrobial Activity of Naesohwangryuntang and Its Composition Oriental Medicins. The Korea Journal of Herbology. 2004;19(4):51-60.
19 An BJ, Lee CE, Son JH, Lee JY, Park TS, Park JM, et al. Antioxidant, Anticancer and Antibacterial Activities of Naesohwangryntang and its Ingredients. The Korea Journal of Herbology. 2005;20(4):17-26.
20 Kim JH, Kim TJ, Kim EH, Kim YM. Inhibitory effects of Wisaengtang on Inflammatory mediators in LPS-induced RAW264.7 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2019;32(3):48-57.   DOI
21 Kim SJ, Kim TJ, Kim EH, Kim YM. Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2020;33(1):1-11.   DOI
22 Perer P. Text of The Immune System / Third edition. Seoul:Lifescience 2011;1-27.
23 Kim IS, Kim SH, Lee KJ, Lee DS, Park JS, Lee NK, et al. Text of Essential Immunology. Seoul:daihak. 2017;28-9.
24 Kim TJ, Kim YM, Kim HT. Effects of Naetakcheongeum-san on Anti-inflammatory Activities in RAW 264.7 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2018;31(1):12-21.   DOI
25 Jeng JH, Wang YJ, Chiang BL, Lee PH, Chan CP, Ho YS, et al. Roles of keratinocyte inflammation in oral cancer: regulating the prostaglndin E2, interleukin-6 and $TNF-{\alpha}$ production of oral epithelial cells by areca nut extract and arecoline. Carcinogenesis. 2003;24(8):1301-15.   DOI
26 Korean pharmacology Textbook Editing Board. Korean pharmacology. Seoul: Shinilbooks. 2010:305-819.
27 Kim HY, Han YM. Anti-inflammatory Effect of Paeoniflorigenone Isolated from Paeoniae Radix. Yakhak Hoeji. 2012;56(1):20-5.
28 National Korean Medicine University Textbook Editing Board. Traditional Herbology. Seoul:Younglimsa. 2008:420-1.
29 Choi WY, Jo MJ, Kim SC, Jung JY. Aucklandiae Radix has Inhibitory effects of pro-inflammatory mediator in LPS-induced RAW 264.7 cell. The Journal of Jeahan Oriental Medical Academy. 2009;7(1):1-12.
30 Lim HS, Kim JH, Ha HK, Seo CS, Shin HK. Comparative Study of the Anti-inflammatory Effects of Menthae Herba from Korea and China. Kor. J. Pharmacogn. 2012;43(3):231-8.
31 Ryu DH, Oh SR, Jung TS, Ryu DS. The Effect of Angelica gigas Nakai Extract and Bacillus Polyfermenticus KJS-2 on Atopic Dermatitis induced by DNCB in mice. J Korean Med. 2017;38(3):30-42.   DOI